Re: question about an article / PB2
thank you, gsgs
.
<dl class="PubmedArticle"><dt class="head">Virology. 2002 Nov 25;303(2):240-52. <script language="JavaScript1.2"><!-- var Menu12490387 = [ ["UseLocalConfig", "jsmenu3Config", "", ""], ["CoreNucleotide" , "window.top.location='http://www.ncbi.nlm.nih.gov/sites/entrez?Db=nuccore&DbFrom=pubmed&Cmd=Link&LinkName=pubmed_nuccore&LinkReadableName=CoreNucleotide&IdsFromResult=12490387&ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVAbstractPlus' ", "", ""], ["Taxonomy via GenBank" , "window.top.location='http://www.ncbi.nlm.nih.gov/sites/entrez?Db=taxonomy&DbFrom=pubmed&Cmd=Link&LinkName=pubmed_taxonomy_entrez&LinkReadableName=Taxonomy%20via%20GenBank&IdsFromResult=12490387&ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVAbstractPlus' ", "", ""], ["Nucleotide" , "window.top.location='http://www.ncbi.nlm.nih.gov/sites/entrez?Db=nucleotide&DbFrom=pubmed&Cmd=Link&LinkName=pubmed_nucleotide&LinkReadableName=Nucleotide&IdsFromResult=12490387&ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVAbstractPlus' ", "", ""], ["Protein" , "window.top.location='http://www.ncbi.nlm.nih.gov/sites/entrez?Db=protein&DbFrom=pubmed&Cmd=Link&LinkName=pubmed_protein&LinkReadableName=Protein&IdsFromResult=12490387&ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVAbstractPlus' ", "", ""], ["LinkOut", "window.top.location='http://www.ncbi.nlm.nih.gov/sites/entrez?Cmd=ShowLinkOut&Db=pubmed&TermToSearch=12490387&ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVAbstractPlus' ", "", ""] ] --></script>
</dt><dd class="abstract">
Differential effect of nucleotide substitutions in the 3' arm of the influenza A virus vRNA promoter on transcription/replication by avian and human polymerase complexes is related to the nature of PB2 amino acid 627.
Crescenzo-Chaigne B,
van der Werf S,
Naffakh N.
Unit? de G?n?tique Mol?culaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, Paris, France.
Using a genetic system that allows the in vivo reconstitution of active ribonucleoproteins, the ability to ensure transcription/replication of a viral-like reporter RNA harboring the G(3) --> A(3), U(5) --> C(5), and C(8) --> U(8) mutations (triple 3-5-8 mutations) in the 3' arm of the promoter was examined with core proteins from human or avian strains of influenza A viruses. The efficiency of transcription/replication of the viral-like RNA with the triple 3-5-8 mutations in COS-1 cells was found to be slightly decreased as compared to the wild-type RNA when the polymerase was derived from a human virus. In contrast, it was found to be considerably increased when the polymerase was derived from an avian virus, in agreement with published observations using the avian A/FPV/Bratislava virus (G. Neumann and G. Hobom, 1995, J. Gen. Virol. 76, 1709-1717). This increase could be attributed to the compensation of the defect in transcription/replication activity in the COS-1 mammalian cell line due to the presence of a glutamic acid at PB2 residue 627, characteristic of avian strains of influenza viruses. Our results thus suggest that PB2 and/or cellular proteins interacting with PB2 could be involved in RNA conformational changes during the process of transcription/replication.
PMID: 12490387 [PubMed - indexed for MEDLINE]
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