tetano
Editor, Senior Moderator
J Virol. 2010 Aug 11. [Epub ahead of print]
Quantitative Proteomic Analyses of Influenza virus-Infected Cultured Human Lung Cells.
Coombs KM, Berard A, Xu W, Krokhin O, Meng X, Cortens JP, Kobasa D, Wilkins J, Brown EG.
Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba R3E 0J6 Canada; Manitoba Centre for Proteomics & Systems Biology, Room 799, 715 McDermot Avenue, Winnipeg, Manitoba, Canada R3E 3P4 Canada; Manitoba Institute of Child Health, Room 513, John Buhler Research Centre, 715 McDermot Avenue, Winnipeg, Manitoba R3E 3P4 Canada; Respiratory Viruses Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba R3E 3R2 Canada; Department of Internal Medicine, Faculty of Medicine, University of Manitoba, Winnipeg, R3E 3P4 Canada; Department of Biochemistry, Microbiology, and Immunology, and Emerging Pathogens Research Centre, University of Ottawa, Ottawa, Ontario, K1H 8M5 Canada.
Abstract
Because they are obligate intracellular parasites, all viruses are exclusively and intimately dependent upon host cells for replication. Viruses, in turn, induce profound changes within cells, including apoptosis, morphological changes, and activation of signaling pathways. Many of these alterations have been analyzed by gene arrays, which measure the cellular "transcriptome". Until recently, it has not been possible to extend comparable types of studies to globally examine all the host cellular proteins, which are the actual effector molecules. We have used SILAC (stable isotope labeling by amino acids in cell culture), combined with high-throughput 2-D HPLC/mass spectrometry, to determine quantitative differences in host proteins after infection of human lung A549 cells with human influenza virus A/PR/8/34 (H1N1) for 24 hours. Of the 4689 identified and measured cytosolic protein pairs, 127 were significantly up-regulated at >95% confidence, 153 were significantly down-regulated at >95% confidence, and a total of 87 proteins were up-regulated or down-regulated more than 5-fold at >99% confidence. Gene ontology and pathway analyses indicated differentially-regulated proteins included those involved in host cell immunity and antigen presentation, cell adhesion, metabolism, protein function, signal transduction, and transcription pathways.
PMID: 20702633 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/20702633
Quantitative Proteomic Analyses of Influenza virus-Infected Cultured Human Lung Cells.
Coombs KM, Berard A, Xu W, Krokhin O, Meng X, Cortens JP, Kobasa D, Wilkins J, Brown EG.
Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba R3E 0J6 Canada; Manitoba Centre for Proteomics & Systems Biology, Room 799, 715 McDermot Avenue, Winnipeg, Manitoba, Canada R3E 3P4 Canada; Manitoba Institute of Child Health, Room 513, John Buhler Research Centre, 715 McDermot Avenue, Winnipeg, Manitoba R3E 3P4 Canada; Respiratory Viruses Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba R3E 3R2 Canada; Department of Internal Medicine, Faculty of Medicine, University of Manitoba, Winnipeg, R3E 3P4 Canada; Department of Biochemistry, Microbiology, and Immunology, and Emerging Pathogens Research Centre, University of Ottawa, Ottawa, Ontario, K1H 8M5 Canada.
Abstract
Because they are obligate intracellular parasites, all viruses are exclusively and intimately dependent upon host cells for replication. Viruses, in turn, induce profound changes within cells, including apoptosis, morphological changes, and activation of signaling pathways. Many of these alterations have been analyzed by gene arrays, which measure the cellular "transcriptome". Until recently, it has not been possible to extend comparable types of studies to globally examine all the host cellular proteins, which are the actual effector molecules. We have used SILAC (stable isotope labeling by amino acids in cell culture), combined with high-throughput 2-D HPLC/mass spectrometry, to determine quantitative differences in host proteins after infection of human lung A549 cells with human influenza virus A/PR/8/34 (H1N1) for 24 hours. Of the 4689 identified and measured cytosolic protein pairs, 127 were significantly up-regulated at >95% confidence, 153 were significantly down-regulated at >95% confidence, and a total of 87 proteins were up-regulated or down-regulated more than 5-fold at >99% confidence. Gene ontology and pathway analyses indicated differentially-regulated proteins included those involved in host cell immunity and antigen presentation, cell adhesion, metabolism, protein function, signal transduction, and transcription pathways.
PMID: 20702633 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/20702633