tetano
Editor, Senior Moderator
Vaccine. 2019 Dec 6. pii: S0264-410X(19)31570-1. doi: 10.1016/j.vaccine.2019.11.041. [Epub ahead of print] [h=1]Quantification of the total neuraminidase content of recent commercially-available influenza vaccines: Introducing a neuraminidase titration reagent.[/h]
Gao Z[SUP]1[/SUP], Robinson K[SUP]1[/SUP], Skowronski DM[SUP]2[/SUP], De Serres G[SUP]3[/SUP], Withers SG[SUP]4[/SUP].
[h=3]Author information[/h] 1 Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, B.C. V6T 1Z1, Canada. 2 School of Population and Public Health, University of British Columbia, 2206 E Mall, Vancouver, British Columbia V6T 1Z3, Canada; Communicable Diseases and Immunization Services, British Columbia Centre for Disease Control, 655 West 12th Ave, Vancouver, British Columbia, Canada. 3 Department of Biological and Occupational Risks, Institut National de Sant? Publique du Qu?bec, Qu?bec, Canada; Department of Social and Preventive Medicine, Laval University, Qu?bec, Canada. 4 Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, B.C. V6T 1Z1, Canada. Electronic address: withers@chem.ubc.ca.
[h=3]Abstract[/h] The protective immunological effects of the influenza neuraminidase (NA) surface protein are of renewed interest but NA content in vaccines remains unstandardized and methods to easily and reliably quantify NA content are unsatisfactory. We describe the use of a recently developed fluorometric titration reagent, TR1, to efficiently quantify the total enzymatically active NA content of six commercially-available influenza vaccines, including split/subunit, inactivated/live and standard /high dose products distributed from 2015/16 to 2017/18 in North America. Considerable differences in active NA content were measured between influenza vaccine products for the same season, with relative content differences between brands generally maintained across seasons. These results highlight the simplicity of use of this reagent, and its unique ability to quantitate enzymatically active NA without the need for specific activities of individual enzymes. The reagent could also prove valuable in assessing the importance of using fully active enzyme to generate protective immune responses.
Copyright ? 2019 Elsevier Ltd. All rights reserved.
[h=4]KEYWORDS:[/h] Active-site titration; HA and NA levels in vaccines; Neuraminidase content
PMID: 31818533 DOI: 10.1016/j.vaccine.2019.11.041
Gao Z[SUP]1[/SUP], Robinson K[SUP]1[/SUP], Skowronski DM[SUP]2[/SUP], De Serres G[SUP]3[/SUP], Withers SG[SUP]4[/SUP].
[h=3]Author information[/h] 1 Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, B.C. V6T 1Z1, Canada. 2 School of Population and Public Health, University of British Columbia, 2206 E Mall, Vancouver, British Columbia V6T 1Z3, Canada; Communicable Diseases and Immunization Services, British Columbia Centre for Disease Control, 655 West 12th Ave, Vancouver, British Columbia, Canada. 3 Department of Biological and Occupational Risks, Institut National de Sant? Publique du Qu?bec, Qu?bec, Canada; Department of Social and Preventive Medicine, Laval University, Qu?bec, Canada. 4 Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, B.C. V6T 1Z1, Canada. Electronic address: withers@chem.ubc.ca.
[h=3]Abstract[/h] The protective immunological effects of the influenza neuraminidase (NA) surface protein are of renewed interest but NA content in vaccines remains unstandardized and methods to easily and reliably quantify NA content are unsatisfactory. We describe the use of a recently developed fluorometric titration reagent, TR1, to efficiently quantify the total enzymatically active NA content of six commercially-available influenza vaccines, including split/subunit, inactivated/live and standard /high dose products distributed from 2015/16 to 2017/18 in North America. Considerable differences in active NA content were measured between influenza vaccine products for the same season, with relative content differences between brands generally maintained across seasons. These results highlight the simplicity of use of this reagent, and its unique ability to quantitate enzymatically active NA without the need for specific activities of individual enzymes. The reagent could also prove valuable in assessing the importance of using fully active enzyme to generate protective immune responses.
Copyright ? 2019 Elsevier Ltd. All rights reserved.
[h=4]KEYWORDS:[/h] Active-site titration; HA and NA levels in vaccines; Neuraminidase content
PMID: 31818533 DOI: 10.1016/j.vaccine.2019.11.041