tetano
Editor, Senior Moderator
J Virol. 2012 Sep 26. [Epub ahead of print]
Pseudotyped Influenza A Virus as a Vaccine for the induction of Heterotypic Immunity.
Powell TJ, Silk JD, Sharps J, Fodor E, Townsend AR.
Source
Molecular Immunology Group.
Abstract
There is a need for vaccines that can protect broadly across all Influenza A strains. We have produced a pseudotyped influenza virus based on suppression of the A/PR/8/34 hemagglutinin signal sequence (S-FLU) that can infect cells and express the viral core proteins and neuraminidase, but cannot replicate. We show that when given by inhalation to mice S-FLU is non-pathogenic, but generates a vigorous T cell response in the lung associated with markedly reduced viral titers and weight loss after challenge with H1 and H3 influenza viruses. These properties of S-FLU suggest that it may have potential as a broadly protective A virus vaccine, particularly in the setting of a threatened pandemic before matched subunit vaccines become available.
PMID:
23015719
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23015719
Pseudotyped Influenza A Virus as a Vaccine for the induction of Heterotypic Immunity.
Powell TJ, Silk JD, Sharps J, Fodor E, Townsend AR.
Source
Molecular Immunology Group.
Abstract
There is a need for vaccines that can protect broadly across all Influenza A strains. We have produced a pseudotyped influenza virus based on suppression of the A/PR/8/34 hemagglutinin signal sequence (S-FLU) that can infect cells and express the viral core proteins and neuraminidase, but cannot replicate. We show that when given by inhalation to mice S-FLU is non-pathogenic, but generates a vigorous T cell response in the lung associated with markedly reduced viral titers and weight loss after challenge with H1 and H3 influenza viruses. These properties of S-FLU suggest that it may have potential as a broadly protective A virus vaccine, particularly in the setting of a threatened pandemic before matched subunit vaccines become available.
PMID:
23015719
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23015719