tetano
Editor, Senior Moderator
Proteomics. 2012 May 23. doi: 10.1002/pmic.201100619. [Epub ahead of print]
Proteomic analysis of the lungs of mice infected with different pathotypes of H5N1 avian influenza viruses.
Zhao D, Liang L, Li Y, Liu L, Guan Y, Jiang Y, Chen H.
Source
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, 427 Maduan Street, Harbin, 150001, P. R. China.
Abstract
The virulence of influenza virus is determined by viral and host factors. Data on the genetic basis of the virulence of H5N1 influenza viruses have increased over the past decade;, however, the contributions of host factors to the outcomes of H5N1 infection remain largely unknown. Here, we tested two chicken H5N1 viruses in mice and found that A/chicken/VN1214/2007 was non-lethal in mice and only replicated in the lung, whereas A/chicken/VN1180/2006 was highly lethal and replicated systemically in mice. To investigate the host response against these two different virus infections, we performed proteomic analysis by using 2-D Fluorescence Difference Gel Electrophoresis on the lung tissues of mice collected on day 1 and day 3 post-inoculation with different viruses or PBS as a control. Thirty-nine differentially expressed (DE) proteins related to "immune and stimulus response", "macromolecular biosynthesis and metabolism", and "cellular component and cytoskeleton" were identified in the virus-inoculated groups. Moreover, 13 DE proteins were identified between the two virus-inoculated groups, implying that these proteins may play important roles in the different outcomes of infection with these two viruses. Our data provide important information regarding the host response to mild and lethal H5N1 influenza virus infection.
? 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PMID:
22623221
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22623221
Proteomic analysis of the lungs of mice infected with different pathotypes of H5N1 avian influenza viruses.
Zhao D, Liang L, Li Y, Liu L, Guan Y, Jiang Y, Chen H.
Source
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, 427 Maduan Street, Harbin, 150001, P. R. China.
Abstract
The virulence of influenza virus is determined by viral and host factors. Data on the genetic basis of the virulence of H5N1 influenza viruses have increased over the past decade;, however, the contributions of host factors to the outcomes of H5N1 infection remain largely unknown. Here, we tested two chicken H5N1 viruses in mice and found that A/chicken/VN1214/2007 was non-lethal in mice and only replicated in the lung, whereas A/chicken/VN1180/2006 was highly lethal and replicated systemically in mice. To investigate the host response against these two different virus infections, we performed proteomic analysis by using 2-D Fluorescence Difference Gel Electrophoresis on the lung tissues of mice collected on day 1 and day 3 post-inoculation with different viruses or PBS as a control. Thirty-nine differentially expressed (DE) proteins related to "immune and stimulus response", "macromolecular biosynthesis and metabolism", and "cellular component and cytoskeleton" were identified in the virus-inoculated groups. Moreover, 13 DE proteins were identified between the two virus-inoculated groups, implying that these proteins may play important roles in the different outcomes of infection with these two viruses. Our data provide important information regarding the host response to mild and lethal H5N1 influenza virus infection.
? 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PMID:
22623221
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22623221