tetano
Editor, Senior Moderator
Proteins
. 2021 Aug 8.
doi: 10.1002/prot.26205. Online ahead of print.
A distinct ssDNA/RNA binding interface in the Nsp9 protein from SARS-CoV-2
Serene El-Kamand[SUP] #[/SUP][SUP] 1 [/SUP], Mar-Dean Du Plessis[SUP] #[/SUP][SUP] 1 [/SUP], Natasha Breen[SUP] 1 [/SUP], Lexie Johnson[SUP] 1 [/SUP], Samuel Beard[SUP] 2 [/SUP], Ann H Kwan[SUP] 3 [/SUP], Derek J Richard[SUP] 2 [/SUP], Liza Cubeddu[SUP] 1 3 [/SUP], Roland Gamsjaeger[SUP] 1 3 [/SUP]
Affiliations
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel, highly infectious RNA virus that belongs to the coronavirus family. Replication of the viral genome is a fundamental step in the virus life cycle and SARS-CoV-2 non-structural protein 9 (Nsp9) is shown to be essential for virus replication through its ability to bind RNA in the closely related SARS-CoV-1 strain. Two recent studies revealing the three-dimensional structure of Nsp9 from SARS-CoV-2 have demonstrated a high degree of similarity between Nsp9 proteins within the coronavirus family. However, the binding affinity to RNA is very low which, until now, has prevented the determination of the structural details of this interaction. In this study, we have utilised nuclear magnetic resonance spectroscopy (NMR) in combination with surface biolayer interferometry (BLI) to reveal a distinct binding interface for both ssDNA and RNA that is different to the one proposed in the recently solved SARS-CoV-2 replication and transcription complex (RTC) structure. Based on these data, we have proposed a structural model of a Nsp9-RNA complex, shedding light on the molecular details of these important interactions.
Keywords: COVID-19; Nsp9; RNA; SARS-CoV-2; coronavirus.
. 2021 Aug 8.
doi: 10.1002/prot.26205. Online ahead of print.
A distinct ssDNA/RNA binding interface in the Nsp9 protein from SARS-CoV-2
Serene El-Kamand[SUP] #[/SUP][SUP] 1 [/SUP], Mar-Dean Du Plessis[SUP] #[/SUP][SUP] 1 [/SUP], Natasha Breen[SUP] 1 [/SUP], Lexie Johnson[SUP] 1 [/SUP], Samuel Beard[SUP] 2 [/SUP], Ann H Kwan[SUP] 3 [/SUP], Derek J Richard[SUP] 2 [/SUP], Liza Cubeddu[SUP] 1 3 [/SUP], Roland Gamsjaeger[SUP] 1 3 [/SUP]
Affiliations
- PMID: 34369011
- DOI: 10.1002/prot.26205
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel, highly infectious RNA virus that belongs to the coronavirus family. Replication of the viral genome is a fundamental step in the virus life cycle and SARS-CoV-2 non-structural protein 9 (Nsp9) is shown to be essential for virus replication through its ability to bind RNA in the closely related SARS-CoV-1 strain. Two recent studies revealing the three-dimensional structure of Nsp9 from SARS-CoV-2 have demonstrated a high degree of similarity between Nsp9 proteins within the coronavirus family. However, the binding affinity to RNA is very low which, until now, has prevented the determination of the structural details of this interaction. In this study, we have utilised nuclear magnetic resonance spectroscopy (NMR) in combination with surface biolayer interferometry (BLI) to reveal a distinct binding interface for both ssDNA and RNA that is different to the one proposed in the recently solved SARS-CoV-2 replication and transcription complex (RTC) structure. Based on these data, we have proposed a structural model of a Nsp9-RNA complex, shedding light on the molecular details of these important interactions.
Keywords: COVID-19; Nsp9; RNA; SARS-CoV-2; coronavirus.