tetano
Editor, Senior Moderator
Protein Sci. 2020 Apr 17. doi: 10.1002/pro.3873. [Epub ahead of print]
Crystal structure of Nsp15 endoribonuclease NendoU from SARS-CoV-2.
Kim Y[SUP]1,[/SUP][SUP]2[/SUP], Jedrzejczak R[SUP]1,[/SUP][SUP]2[/SUP], Maltseva NI[SUP]1,[/SUP][SUP]2[/SUP], Wilamowski M[SUP]1,[/SUP][SUP]3[/SUP], Endres M[SUP]2[/SUP], Godzik A[SUP]4[/SUP], Michalska K[SUP]1,[/SUP][SUP]2[/SUP], Joachimiak A[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP].
Author information
Abstract
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is rapidly spreading around the world. There is no existing vaccine or proven drug to prevent infections and stop virus proliferation. Although this virus is similar to human and animal SARS- and MERS-CoVs, the detailed information about SARS-CoV-2 proteins structures and functions is urgently needed to rapidly develop effective vaccines, antibodies and antivirals. We applied high-throughput protein production and structure determination pipeline at the Center for Structural Genomics of Infectious Diseases to produce SARS-CoV-2 proteins and structures. Here we report two high-resolution crystal structures of endoribonuclease Nsp15/NendoU. We compare these structures with previously reported homologs from SARS and MERS coronaviruses. This article is protected by copyright. All rights reserved.
? 2020 The Protein Society.
KEYWORDS:
COVID-19; EndoU family; NendoU; Nsp15; SARS-CoV-2; crystal structure; endoribonuclease
PMID:32304108DOI:10.1002/pro.3873
Crystal structure of Nsp15 endoribonuclease NendoU from SARS-CoV-2.
Kim Y[SUP]1,[/SUP][SUP]2[/SUP], Jedrzejczak R[SUP]1,[/SUP][SUP]2[/SUP], Maltseva NI[SUP]1,[/SUP][SUP]2[/SUP], Wilamowski M[SUP]1,[/SUP][SUP]3[/SUP], Endres M[SUP]2[/SUP], Godzik A[SUP]4[/SUP], Michalska K[SUP]1,[/SUP][SUP]2[/SUP], Joachimiak A[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP].
Author information
Abstract
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is rapidly spreading around the world. There is no existing vaccine or proven drug to prevent infections and stop virus proliferation. Although this virus is similar to human and animal SARS- and MERS-CoVs, the detailed information about SARS-CoV-2 proteins structures and functions is urgently needed to rapidly develop effective vaccines, antibodies and antivirals. We applied high-throughput protein production and structure determination pipeline at the Center for Structural Genomics of Infectious Diseases to produce SARS-CoV-2 proteins and structures. Here we report two high-resolution crystal structures of endoribonuclease Nsp15/NendoU. We compare these structures with previously reported homologs from SARS and MERS coronaviruses. This article is protected by copyright. All rights reserved.
? 2020 The Protein Society.
KEYWORDS:
COVID-19; EndoU family; NendoU; Nsp15; SARS-CoV-2; crystal structure; endoribonuclease
PMID:32304108DOI:10.1002/pro.3873