tetano
Editor, Senior Moderator
Vet Microbiol. 2018 Sep;223:21-26. doi: 10.1016/j.vetmic.2018.07.011. Epub 2018 Jul 17.
[h=1]Protective efficacy of an inactivated chimeric H7/H5 avian influenza vaccine against highly pathogenic avian influenza H7N9 and clade 2.3.4.4 H5 viruses.[/h] Peng C[SUP]1[/SUP], Hou G[SUP]1[/SUP], Li J[SUP]1[/SUP], Wang S[SUP]1[/SUP], Wang Y[SUP]2[/SUP], Cheng S[SUP]1[/SUP], Yu X[SUP]1[/SUP], Jin J[SUP]1[/SUP], Jiang W[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The highly pathogenic avian influenza (HPAI) H5 and H7N9 viruses pose a serious challenge to public health and the poultry industry in China. In this study, we generated a chimeric H7/H5 recombinant virus that expressed the entire HA1 region of the HPAI A/chicken/Guangdong/RZ/2017(H7N9) virus and the HA2 region of the HPAI A/chicken/Fujian/5/2016(H5N6) viruses. The resulting chimeric PR8-H7/H5 virus exhibited similar growth kinetics as the parental PR8-H5 and PR8-H7 viruses in vitro. The inactivated chimeric PR8-H7/H5 vaccine induced specific, cross-reactive hemagglutination inhibition antibodies against the H7 virus only but induced serum-neutralizing antibodies against both H7 and H5 viruses. Furthermore, the inactivated chimeric PR8-H7/H5 vaccine significantly reduced virus shedding and protected chickens from challenge with the HPAI H5N6 and H7N9 viruses. Our results suggested that the inactivated chimeric PR8-H7/H5 vaccine was effective against HPAI H5 and H7N9 viruses in chickens.
[h=4]KEYWORDS:[/h] Avian influenza virus; Chimeric vaccine; H5N6; H7N9
PMID: 30173747 DOI: 10.1016/j.vetmic.2018.07.011
[h=1]Protective efficacy of an inactivated chimeric H7/H5 avian influenza vaccine against highly pathogenic avian influenza H7N9 and clade 2.3.4.4 H5 viruses.[/h] Peng C[SUP]1[/SUP], Hou G[SUP]1[/SUP], Li J[SUP]1[/SUP], Wang S[SUP]1[/SUP], Wang Y[SUP]2[/SUP], Cheng S[SUP]1[/SUP], Yu X[SUP]1[/SUP], Jin J[SUP]1[/SUP], Jiang W[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The highly pathogenic avian influenza (HPAI) H5 and H7N9 viruses pose a serious challenge to public health and the poultry industry in China. In this study, we generated a chimeric H7/H5 recombinant virus that expressed the entire HA1 region of the HPAI A/chicken/Guangdong/RZ/2017(H7N9) virus and the HA2 region of the HPAI A/chicken/Fujian/5/2016(H5N6) viruses. The resulting chimeric PR8-H7/H5 virus exhibited similar growth kinetics as the parental PR8-H5 and PR8-H7 viruses in vitro. The inactivated chimeric PR8-H7/H5 vaccine induced specific, cross-reactive hemagglutination inhibition antibodies against the H7 virus only but induced serum-neutralizing antibodies against both H7 and H5 viruses. Furthermore, the inactivated chimeric PR8-H7/H5 vaccine significantly reduced virus shedding and protected chickens from challenge with the HPAI H5N6 and H7N9 viruses. Our results suggested that the inactivated chimeric PR8-H7/H5 vaccine was effective against HPAI H5 and H7N9 viruses in chickens.
[h=4]KEYWORDS:[/h] Avian influenza virus; Chimeric vaccine; H5N6; H7N9
PMID: 30173747 DOI: 10.1016/j.vetmic.2018.07.011