tetano
Editor, Senior Moderator
Virus Res. 2015 Jan 16. pii: S0168-1702(15)00015-5. doi: 10.1016/j.virusres.2015.01.009. [Epub ahead of print]
[h=1]Protection of pigs against pandemic swine origin H1N1 influenza A virus infection by hemagglutinin- or neuraminidase-expressing attenuated pseudorabies virus recombinants.[/h] Klingbeil K[SUP]1[/SUP], Lange E[SUP]2[/SUP], Blohm U[SUP]3[/SUP], Teifke JP[SUP]2[/SUP], Mettenleiter TC[SUP]1[/SUP], Fuchs W[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza is an important respiratory disease of pigs, and may lead to novel human pathogens like the 2009 pandemic H1N1 swine-origin influenza virus (SoIV). Therefore, improved influenza vaccines for pigs are required. Recently, we demonstrated that single intranasal immunization with a hemagglutinin (HA)-expressing pseudorabies virus recombinant of vaccine strain Bartha (PrV-Ba) protected pigs from H1N1 SoIV challenge (Klingbeil, K., Lange, E., Teifke, J.P., Mettenleiter, T.C., Fuchs, W., 2014. Immunization of pigs with an attenuated pseudorabies virus recombinant expressing the haemagglutinin of pandemic swine origin H1N1 influenza A virus. J. Gen. Virol. 95, 948-959). Now we investigated enhancement of efficacy by prime-boost vaccination and/or intramuscular administration. Furthermore, a novel PrV-Ba recombinant expressing codon-optimized N1 neuraminidase (NA) was included. In vitro replication of this virus was only slightly affected compared to parental virus. Unlike HA, the abundantly expressed NA was efficiently incorporated into PrV particles. Immunization of pigs with the two PrV recombinants, either singly or in combination, induced B cell proliferation and the expected SoIV-specific antibodies, whose titers increased substantially after boost vaccination. Animals immunized with either PrV recombinant were protected from disease after challenge with H1N1 SoIV, and challenge virus replication was significantly reduced compared to PrV-Ba vaccinated or na?ve controls. Protective efficacy of HA-expressing PrV was higher than of NA-expressing PrV, and not significantly enhanced by combination. Despite higher serum antibody titers obtained after intramuscular immunization, transmission of challenge virus to na?ve contact animals was only prevented after intranasal prime-boost vaccination with HA-expressing PrV-Ba.
Copyright ? 2015. Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] Pandemic H1N1 SoIV; attenuated PrV strain Bartha; hemagglutinin (HA); neuraminidase (NA); vectored vaccine
PMID: 25599604 [PubMed - as supplied by publisher]
[h=1]Protection of pigs against pandemic swine origin H1N1 influenza A virus infection by hemagglutinin- or neuraminidase-expressing attenuated pseudorabies virus recombinants.[/h] Klingbeil K[SUP]1[/SUP], Lange E[SUP]2[/SUP], Blohm U[SUP]3[/SUP], Teifke JP[SUP]2[/SUP], Mettenleiter TC[SUP]1[/SUP], Fuchs W[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza is an important respiratory disease of pigs, and may lead to novel human pathogens like the 2009 pandemic H1N1 swine-origin influenza virus (SoIV). Therefore, improved influenza vaccines for pigs are required. Recently, we demonstrated that single intranasal immunization with a hemagglutinin (HA)-expressing pseudorabies virus recombinant of vaccine strain Bartha (PrV-Ba) protected pigs from H1N1 SoIV challenge (Klingbeil, K., Lange, E., Teifke, J.P., Mettenleiter, T.C., Fuchs, W., 2014. Immunization of pigs with an attenuated pseudorabies virus recombinant expressing the haemagglutinin of pandemic swine origin H1N1 influenza A virus. J. Gen. Virol. 95, 948-959). Now we investigated enhancement of efficacy by prime-boost vaccination and/or intramuscular administration. Furthermore, a novel PrV-Ba recombinant expressing codon-optimized N1 neuraminidase (NA) was included. In vitro replication of this virus was only slightly affected compared to parental virus. Unlike HA, the abundantly expressed NA was efficiently incorporated into PrV particles. Immunization of pigs with the two PrV recombinants, either singly or in combination, induced B cell proliferation and the expected SoIV-specific antibodies, whose titers increased substantially after boost vaccination. Animals immunized with either PrV recombinant were protected from disease after challenge with H1N1 SoIV, and challenge virus replication was significantly reduced compared to PrV-Ba vaccinated or na?ve controls. Protective efficacy of HA-expressing PrV was higher than of NA-expressing PrV, and not significantly enhanced by combination. Despite higher serum antibody titers obtained after intramuscular immunization, transmission of challenge virus to na?ve contact animals was only prevented after intranasal prime-boost vaccination with HA-expressing PrV-Ba.
Copyright ? 2015. Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] Pandemic H1N1 SoIV; attenuated PrV strain Bartha; hemagglutinin (HA); neuraminidase (NA); vectored vaccine
PMID: 25599604 [PubMed - as supplied by publisher]