tetano
Editor, Senior Moderator
Diagn Microbiol Infect Dis. 2014 Jan 30. pii: S0732-8893(14)00051-0. doi: 10.1016/j.diagmicrobio.2014.01.018. [Epub ahead of print]
Prompt detection of influenza A and B viruses using the BD Veritor? System Flu A+B, Quidel? Sofia? Influenza A+B FIA, and Alere BinaxNOW? Influenza A&B compared to real-time reverse transcription-polymerase chain reaction (RT-PCR).
Dunn J1, Obuekwe J2, Baun T2, Rogers J2, Patel T2, Snow L2.
Author information
Abstract
The performance characteristics of rapid influenza diagnostic tests vary widely. This study evaluated the BD Veritor? System Flu A+B (Veritor; BD Diagnostics, Sparks, MD, USA), Quidel? Sofia? Influenza A+B FIA (Sofia; Quidel Corp., San Diego, CA, USA), and Alere BinaxNOW? Influenza A&B (Binax; Alere Scarborough, Inc., Scarborough, ME, USA) compared to reverse transcription-polymerase chain reaction (RT-PCR) for detection of influenza viruses in nasal wash specimens from 240 pediatric patients. Positive percent agreements for influenza A and B virus detection were 93.8% and 94.2%, 95.8% and 98.1%, and 79.2% and 80.8% for Veritor, Sofia, and Binax, respectively. The Veritor and Binax tests demonstrated negative percent agreements >97.9% for detection of both influenza viruses, but the negative percent agreement of the Sofia test was 91.1% for influenza A and 70.7% for influenza B virus. Overall, the Veritor and Sofia tests were nearly as sensitive as RT-PCR and considerably more sensitive than Binax for detection of influenza viruses. However, the accuracy of the Sofia test was significantly lower than either Veritor or Binax.
Copyright ? 2014 The Authors. Published by Elsevier Inc. All rights reserved.
KEYWORDS:
Influenza viruses, RT-PCR, Rapid diagnostics, Workflow analysis
PMID:
24582581
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24582581
Prompt detection of influenza A and B viruses using the BD Veritor? System Flu A+B, Quidel? Sofia? Influenza A+B FIA, and Alere BinaxNOW? Influenza A&B compared to real-time reverse transcription-polymerase chain reaction (RT-PCR).
Dunn J1, Obuekwe J2, Baun T2, Rogers J2, Patel T2, Snow L2.
Author information
Abstract
The performance characteristics of rapid influenza diagnostic tests vary widely. This study evaluated the BD Veritor? System Flu A+B (Veritor; BD Diagnostics, Sparks, MD, USA), Quidel? Sofia? Influenza A+B FIA (Sofia; Quidel Corp., San Diego, CA, USA), and Alere BinaxNOW? Influenza A&B (Binax; Alere Scarborough, Inc., Scarborough, ME, USA) compared to reverse transcription-polymerase chain reaction (RT-PCR) for detection of influenza viruses in nasal wash specimens from 240 pediatric patients. Positive percent agreements for influenza A and B virus detection were 93.8% and 94.2%, 95.8% and 98.1%, and 79.2% and 80.8% for Veritor, Sofia, and Binax, respectively. The Veritor and Binax tests demonstrated negative percent agreements >97.9% for detection of both influenza viruses, but the negative percent agreement of the Sofia test was 91.1% for influenza A and 70.7% for influenza B virus. Overall, the Veritor and Sofia tests were nearly as sensitive as RT-PCR and considerably more sensitive than Binax for detection of influenza viruses. However, the accuracy of the Sofia test was significantly lower than either Veritor or Binax.
Copyright ? 2014 The Authors. Published by Elsevier Inc. All rights reserved.
KEYWORDS:
Influenza viruses, RT-PCR, Rapid diagnostics, Workflow analysis
PMID:
24582581
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24582581