tetano
Editor, Senior Moderator
J Infect Dis. 2014 Nov 2. pii: jiu573. [Epub ahead of print]
Prolonged Pro-Inflammatory Cytokine Production in Monocytes Following Influenza Vaccination is Modulated by IL-10 in Older Adults.
Mohanty S1, Joshi SR1, Ueda I1, Wilson J1, Blevins T2, Siconolfi B1, Meng H3, Devine L4, Raddassi K5, Tsang S1, Belshe RB2, Hafler DA4, Kaech SM6, Kleinstein SH7, Trentalange M8, Allore HG8, Shaw AC1.
Author information
Abstract
We evaluated in vivo innate immune responses in monocyte populations from 67 young (age 21-30) and older (age≥65) adults prior to and following influenza vaccination. We found that CD14+ CD16+ inflammatory monocytes were induced following vaccination in both young and older adults. In classical CD14+ CD16- and inflammatory monocytes, TNF-α and IL-6 production as measured by intracellular staining were strongly induced following vaccination. Cytokine production was strongly associated with influenza vaccine antibody response; highest levels were found as late as day 28 post-vaccine in young subjects, and were substantially diminished in older subjects. Notably, levels of the anti-inflammatory cytokine IL-10 were markedly elevated in monocytes from older subjects pre- and post-vaccine. In purified monocytes, we found age-associated elevation in phosphorylated STAT3, and decreased serine 359-phosphorylation of the negative IL-10 regulator DUSP1. These findings for the first time implicate dysregulated IL-10 production in impaired vaccine responses in older adults.
? The Author 2014. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.
PMID:
25367297
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25367297
http://www.ncbi.nlm.nih.gov/pubmed/25367297
Prolonged Pro-Inflammatory Cytokine Production in Monocytes Following Influenza Vaccination is Modulated by IL-10 in Older Adults.
Mohanty S1, Joshi SR1, Ueda I1, Wilson J1, Blevins T2, Siconolfi B1, Meng H3, Devine L4, Raddassi K5, Tsang S1, Belshe RB2, Hafler DA4, Kaech SM6, Kleinstein SH7, Trentalange M8, Allore HG8, Shaw AC1.
Author information
Abstract
We evaluated in vivo innate immune responses in monocyte populations from 67 young (age 21-30) and older (age≥65) adults prior to and following influenza vaccination. We found that CD14+ CD16+ inflammatory monocytes were induced following vaccination in both young and older adults. In classical CD14+ CD16- and inflammatory monocytes, TNF-α and IL-6 production as measured by intracellular staining were strongly induced following vaccination. Cytokine production was strongly associated with influenza vaccine antibody response; highest levels were found as late as day 28 post-vaccine in young subjects, and were substantially diminished in older subjects. Notably, levels of the anti-inflammatory cytokine IL-10 were markedly elevated in monocytes from older subjects pre- and post-vaccine. In purified monocytes, we found age-associated elevation in phosphorylated STAT3, and decreased serine 359-phosphorylation of the negative IL-10 regulator DUSP1. These findings for the first time implicate dysregulated IL-10 production in impaired vaccine responses in older adults.
? The Author 2014. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.
PMID:
25367297
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25367297
http://www.ncbi.nlm.nih.gov/pubmed/25367297