tetano
Editor, Senior Moderator
Sci Immunol. 2019 Apr 19;4(34). pii: eaau2710. doi: 10.1126/sciimmunol.aau2710.
[h=1]Prolonged evolution of the memory B cell response induced by a replicating adenovirus-influenza H5 vaccine.[/h] Matsuda K[SUP]1[/SUP], Huang J[SUP]1[/SUP], Zhou T[SUP]2[/SUP], Sheng Z[SUP]3[/SUP], Kang BH[SUP]1[/SUP], Ishida E[SUP]1[/SUP], Griesman T[SUP]1[/SUP], Stuccio S[SUP]1[/SUP], Bolkhovitinov L[SUP]1[/SUP], Wohlbold TJ[SUP]4[/SUP], Chromikova V[SUP]4[/SUP], Cagigi A[SUP]2[/SUP], Leung K[SUP]2[/SUP], Andrews S[SUP]2[/SUP], Cheung CSF[SUP]2[/SUP], Pullano AA[SUP]1[/SUP], Plyler J[SUP]2[/SUP], Soto C[SUP]2[/SUP], Zhang B[SUP]2[/SUP], Yang Y[SUP]2[/SUP], Joyce MG[SUP]2[/SUP], Tsybovsky Y[SUP]5[/SUP], Wheatley A[SUP]2[/SUP], Narpala SR[SUP]2[/SUP], Guo Y[SUP]3[/SUP], Darko S[SUP]2[/SUP], Bailer RT[SUP]2[/SUP], Poole A[SUP]1[/SUP], Liang CJ[SUP]6[/SUP], Smith J[SUP]7[/SUP], Alexander J[SUP]7[/SUP], Gurwith M[SUP]7[/SUP], Migueles SA[SUP]1[/SUP], Koup RA[SUP]2[/SUP], Golding H[SUP]8[/SUP], Khurana S[SUP]8[/SUP], McDermott AB[SUP]2[/SUP], Shapiro L[SUP]3[/SUP], Krammer F[SUP]4[/SUP], Kwong PD[SUP]2[/SUP], Connors M[SUP]9[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Induction of an antibody response capable of recognizing highly diverse strains is a major obstacle to the development of vaccines for viruses such as HIV and influenza. Here, we report the dynamics of B cell expansion and evolution at the single-cell level after vaccination with a replication-competent adenovirus type 4 recombinant virus expressing influenza H5 hemagglutinin. Fluorescent H1 or H5 probes were used to quantitate and isolate peripheral blood B cells and their antigen receptors. We observed increases in H5-specific antibody somatic hypermutation and potency for several months beyond the period of active viral replication that was not detectable at the serum level. Individual broad and potent antibodies could be isolated, including one stem-specific antibody that is part of a new multidonor class. These results demonstrate prolonged evolution of the B cell response for months after vaccination and should be considered in efforts to evaluate or boost vaccine-induced immunity.
Copyright ? 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
PMID: 31004012 DOI: 10.1126/sciimmunol.aau2710
[h=1]Prolonged evolution of the memory B cell response induced by a replicating adenovirus-influenza H5 vaccine.[/h] Matsuda K[SUP]1[/SUP], Huang J[SUP]1[/SUP], Zhou T[SUP]2[/SUP], Sheng Z[SUP]3[/SUP], Kang BH[SUP]1[/SUP], Ishida E[SUP]1[/SUP], Griesman T[SUP]1[/SUP], Stuccio S[SUP]1[/SUP], Bolkhovitinov L[SUP]1[/SUP], Wohlbold TJ[SUP]4[/SUP], Chromikova V[SUP]4[/SUP], Cagigi A[SUP]2[/SUP], Leung K[SUP]2[/SUP], Andrews S[SUP]2[/SUP], Cheung CSF[SUP]2[/SUP], Pullano AA[SUP]1[/SUP], Plyler J[SUP]2[/SUP], Soto C[SUP]2[/SUP], Zhang B[SUP]2[/SUP], Yang Y[SUP]2[/SUP], Joyce MG[SUP]2[/SUP], Tsybovsky Y[SUP]5[/SUP], Wheatley A[SUP]2[/SUP], Narpala SR[SUP]2[/SUP], Guo Y[SUP]3[/SUP], Darko S[SUP]2[/SUP], Bailer RT[SUP]2[/SUP], Poole A[SUP]1[/SUP], Liang CJ[SUP]6[/SUP], Smith J[SUP]7[/SUP], Alexander J[SUP]7[/SUP], Gurwith M[SUP]7[/SUP], Migueles SA[SUP]1[/SUP], Koup RA[SUP]2[/SUP], Golding H[SUP]8[/SUP], Khurana S[SUP]8[/SUP], McDermott AB[SUP]2[/SUP], Shapiro L[SUP]3[/SUP], Krammer F[SUP]4[/SUP], Kwong PD[SUP]2[/SUP], Connors M[SUP]9[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Induction of an antibody response capable of recognizing highly diverse strains is a major obstacle to the development of vaccines for viruses such as HIV and influenza. Here, we report the dynamics of B cell expansion and evolution at the single-cell level after vaccination with a replication-competent adenovirus type 4 recombinant virus expressing influenza H5 hemagglutinin. Fluorescent H1 or H5 probes were used to quantitate and isolate peripheral blood B cells and their antigen receptors. We observed increases in H5-specific antibody somatic hypermutation and potency for several months beyond the period of active viral replication that was not detectable at the serum level. Individual broad and potent antibodies could be isolated, including one stem-specific antibody that is part of a new multidonor class. These results demonstrate prolonged evolution of the B cell response for months after vaccination and should be considered in efforts to evaluate or boost vaccine-induced immunity.
Copyright ? 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
PMID: 31004012 DOI: 10.1126/sciimmunol.aau2710