tetano
Editor, Senior Moderator
J Vet Res. 2019 Oct 24;63(4):489-495. doi: 10.2478/jvetres-2019-0063. eCollection 2019 Dec. Proinflammatory Cytokine Changes in Bronchoalveolar Lavage Fluid Cells Isolated from Pigs Infected Solely with Porcine Reproductive and Respiratory Syndrome Virus or Co-infected with Swine Influenza Virus.
Czyżewska-Dors E[SUP]1[/SUP], Pomorska-M?l M[SUP]2[/SUP], Dors A[SUP]1[/SUP], Pluta A[SUP]3[/SUP], Podg?rska K[SUP]1[/SUP], Kwit K[SUP]1[/SUP], Stasiak E[SUP]1[/SUP], Łukomska A[SUP]2[/SUP].
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Abstract
Introduction:
The study evaluated the patterns of local innate immune response in bronchoalveolar lavage fluid (BALF) cells of pigs infected with porcine reproductive and respiratory syndrome virus (PRRSV) alone or co-infected with swine influenza virus (SIV).
Material and Methods:
The study was performed on 26 seven-week-old pigs in three groups: PRRSV-infected (n = 11), PRRSV and SIV-infected (n = 11), and control (n = 4). BALF was collected post euthanasia at 2 and 4 dpi (three piglets per inoculated group) and at 21 dpi (all remaining pigs). Expression of IFN-α, IFN-γ, IL-1β, IL-6, IL-8, and IL-10 mRNA was quantified in BALF cells. PRRSV RNA was quantified in BALF samples using a commercial real-time RT-PCR kit.
Results:
The three cytokines IFN-α, IFN-γ, and IL-1β presented significant expression changes in all experimental pigs. In PRRSV-infected animals IL-8 also did, but in co-infected subjects IL-6 and IL-10 were the additional upregulated cytokines. The highest number of differentially expressed genes was observed at 4 dpi, and significant differences in cytokine gene expression did not occur between the experimental groups at any other time point. The mean PRRSV load in the BALF of PRRSV-infected pigs was higher than that of co-infected pigs at each time point, having statistical significance only at 4 dpi.
Conclusion:
The results of the study indicate that infection with PRRSV alone as well as with SIV interferes with innate and adaptive immune response in the infected host. They also showed that co-infection demonstrates additive effects on IL-6 and IL-10 mRNA expression levels.
? 2019 E. Czyżewska-Dors et al., published by Sciend
J Vet Res. 2019 Oct 24;63(4):489-495. doi: 10.2478/jvetres-2019-0063. eCollection 2019 Dec.
Proinflammatory Cytokine Changes in Bronchoalveolar Lavage Fluid Cells Isolated from Pigs Infected Solely with Porcine Reproductive and Respiratory Syndrome Virus or Co-infected with Swine Influenza Virus.
Czyżewska-Dors E[SUP]1[/SUP], Pomorska-M?l M[SUP]2[/SUP], Dors A[SUP]1[/SUP], Pluta A[SUP]3[/SUP], Podg?rska K[SUP]1[/SUP], Kwit K[SUP]1[/SUP], Stasiak E[SUP]1[/SUP], Łukomska A[SUP]2[/SUP].
Author information
KEYWORDS:
cytokines; local immunity; pigs, porcine reproductive and respiratory syndrome virus; swine influenza virus
PMID: 31934657 PMCID: PMC6950433 DOI: 10.2478/jvetres-2019-0063
Free PMC Article
o.
Czyżewska-Dors E[SUP]1[/SUP], Pomorska-M?l M[SUP]2[/SUP], Dors A[SUP]1[/SUP], Pluta A[SUP]3[/SUP], Podg?rska K[SUP]1[/SUP], Kwit K[SUP]1[/SUP], Stasiak E[SUP]1[/SUP], Łukomska A[SUP]2[/SUP].
Author information
Abstract
Introduction:
The study evaluated the patterns of local innate immune response in bronchoalveolar lavage fluid (BALF) cells of pigs infected with porcine reproductive and respiratory syndrome virus (PRRSV) alone or co-infected with swine influenza virus (SIV).
Material and Methods:
The study was performed on 26 seven-week-old pigs in three groups: PRRSV-infected (n = 11), PRRSV and SIV-infected (n = 11), and control (n = 4). BALF was collected post euthanasia at 2 and 4 dpi (three piglets per inoculated group) and at 21 dpi (all remaining pigs). Expression of IFN-α, IFN-γ, IL-1β, IL-6, IL-8, and IL-10 mRNA was quantified in BALF cells. PRRSV RNA was quantified in BALF samples using a commercial real-time RT-PCR kit.
Results:
The three cytokines IFN-α, IFN-γ, and IL-1β presented significant expression changes in all experimental pigs. In PRRSV-infected animals IL-8 also did, but in co-infected subjects IL-6 and IL-10 were the additional upregulated cytokines. The highest number of differentially expressed genes was observed at 4 dpi, and significant differences in cytokine gene expression did not occur between the experimental groups at any other time point. The mean PRRSV load in the BALF of PRRSV-infected pigs was higher than that of co-infected pigs at each time point, having statistical significance only at 4 dpi.
Conclusion:
The results of the study indicate that infection with PRRSV alone as well as with SIV interferes with innate and adaptive immune response in the infected host. They also showed that co-infection demonstrates additive effects on IL-6 and IL-10 mRNA expression levels.
? 2019 E. Czyżewska-Dors et al., published by Sciend
J Vet Res. 2019 Oct 24;63(4):489-495. doi: 10.2478/jvetres-2019-0063. eCollection 2019 Dec.
Proinflammatory Cytokine Changes in Bronchoalveolar Lavage Fluid Cells Isolated from Pigs Infected Solely with Porcine Reproductive and Respiratory Syndrome Virus or Co-infected with Swine Influenza Virus.
Czyżewska-Dors E[SUP]1[/SUP], Pomorska-M?l M[SUP]2[/SUP], Dors A[SUP]1[/SUP], Pluta A[SUP]3[/SUP], Podg?rska K[SUP]1[/SUP], Kwit K[SUP]1[/SUP], Stasiak E[SUP]1[/SUP], Łukomska A[SUP]2[/SUP].
Author information
KEYWORDS:
cytokines; local immunity; pigs, porcine reproductive and respiratory syndrome virus; swine influenza virus
PMID: 31934657 PMCID: PMC6950433 DOI: 10.2478/jvetres-2019-0063
Free PMC Article
o.