tetano
Editor, Senior Moderator
Appl Microbiol Biotechnol. 2012 Sep 4. [Epub ahead of print]
Production of influenza H1N1 vaccine from MDCK cells using a novel disposable packed-bed bioreactor.
Sun B, Yu X, Kong W, Sun S, Yang P, Zhu C, Zhang H, Wu Y, Chen Y, Shi Y, Zhang X, Jiang C.
Source
Nation Engineering Laboratory for AIDS Vaccine, Jilin University, Changchun, 130012, China.
Abstract
A process for human influenza H1N1 virus vaccine production from Madin-Darby canine kidney (MDCK) cells using a novel packed-bed bioreactor is described in this report. The mini-bioreactor was used to study the relationship between cell density and glucose consumption rate and to optimize the infection parameters of the influenza H1N1 virus (A/New Caledonia/20/99). The MDCK cell culture and virus infection were then monitored in a disposable perfusion bioreactor (AmProtein Current Perfusion Bioreactor) with proportional-integral-derivative control of pH, dissolved O(2) (DO), agitation, and temperature. During 6 days of culture, the total cell number increased from 2.0 ? 10(9) to 3.2 ? 10(10) cells. The maximum virus titers of 768 hemagglutinin units/100 μL and 7.8 ? 10(7) 50 % tissue culture infectious doses/mL were obtained 3 days after infection. These results demonstrate that using a disposable perfusion bioreactor for large-scale cultivation of MDCK cells, which allows for the control of DO, pH, and other conditions, is a convenient and stable platform for industrial-scale production of influenza vaccines.
PMID:
22945265
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22945265
Production of influenza H1N1 vaccine from MDCK cells using a novel disposable packed-bed bioreactor.
Sun B, Yu X, Kong W, Sun S, Yang P, Zhu C, Zhang H, Wu Y, Chen Y, Shi Y, Zhang X, Jiang C.
Source
Nation Engineering Laboratory for AIDS Vaccine, Jilin University, Changchun, 130012, China.
Abstract
A process for human influenza H1N1 virus vaccine production from Madin-Darby canine kidney (MDCK) cells using a novel packed-bed bioreactor is described in this report. The mini-bioreactor was used to study the relationship between cell density and glucose consumption rate and to optimize the infection parameters of the influenza H1N1 virus (A/New Caledonia/20/99). The MDCK cell culture and virus infection were then monitored in a disposable perfusion bioreactor (AmProtein Current Perfusion Bioreactor) with proportional-integral-derivative control of pH, dissolved O(2) (DO), agitation, and temperature. During 6 days of culture, the total cell number increased from 2.0 ? 10(9) to 3.2 ? 10(10) cells. The maximum virus titers of 768 hemagglutinin units/100 μL and 7.8 ? 10(7) 50 % tissue culture infectious doses/mL were obtained 3 days after infection. These results demonstrate that using a disposable perfusion bioreactor for large-scale cultivation of MDCK cells, which allows for the control of DO, pH, and other conditions, is a convenient and stable platform for industrial-scale production of influenza vaccines.
PMID:
22945265
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22945265