Giuseppe
Emeritus
Proc Natl Acad Sci U S A. 2009 Apr 3. [Epub ahead of print]
Transience of MHC Class I-restricted antigen presentation after influenza A virus infection.
Mintern JD, Bedoui S, Davey GM, Moffat JM, Doherty PC, Turner SJ. - Department of Microbiology and Immunology, University of Melbourne, Parkville 3010, Australia;
Antigen expressed as MHC Class I glycoprotein (pMHCI) complexes on dendritic cells is the primary driver of CD8(+) T cell clonal expansion and differentiation. As we seek to define the molecular differences between acutely stimulated cytotoxic T lymphocyte (CTL) effectors and long-lived memory T cells, it is essential that we understand the duration of in vivo pMHCI persistence.
Although infectious influenza A virus is readily cleared by mammalian hosts, that does not necessarily mean that all influenza antigen is totally eliminated. An exhaustive series of carefully controlled adoptive transfer experiments using 3 different carboxy fluorescein diacetate succinimidyl ester-labeled T cell receptor-transgenic CTL populations and a spectrum of genetically engineered and wild-type influenza A viruses provided no evidence for pMHCI persistence over the 30-60-d interval after virus challenge.
Molecular profiles identified in antigen-specific T cells at this time may thus be considered to reflect established immunologic memory and not recent CTL activation from a persistent pMHCI pool.
PMID: 19346476 [PubMed - as supplied by publisher]
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Transience of MHC Class I-restricted antigen presentation after influenza A virus infection.
Mintern JD, Bedoui S, Davey GM, Moffat JM, Doherty PC, Turner SJ. - Department of Microbiology and Immunology, University of Melbourne, Parkville 3010, Australia;
Antigen expressed as MHC Class I glycoprotein (pMHCI) complexes on dendritic cells is the primary driver of CD8(+) T cell clonal expansion and differentiation. As we seek to define the molecular differences between acutely stimulated cytotoxic T lymphocyte (CTL) effectors and long-lived memory T cells, it is essential that we understand the duration of in vivo pMHCI persistence.
Although infectious influenza A virus is readily cleared by mammalian hosts, that does not necessarily mean that all influenza antigen is totally eliminated. An exhaustive series of carefully controlled adoptive transfer experiments using 3 different carboxy fluorescein diacetate succinimidyl ester-labeled T cell receptor-transgenic CTL populations and a spectrum of genetically engineered and wild-type influenza A viruses provided no evidence for pMHCI persistence over the 30-60-d interval after virus challenge.
Molecular profiles identified in antigen-specific T cells at this time may thus be considered to reflect established immunologic memory and not recent CTL activation from a persistent pMHCI pool.
PMID: 19346476 [PubMed - as supplied by publisher]
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