tetano
Editor, Senior Moderator
Proc Natl Acad Sci U S A
. 2022 Jul 5;119(27):e2200109119.
doi: 10.1073/pnas.2200109119. Epub 2022 Jun 28.
The dynamics of SARS-CoV-2 infectivity with changes in aerosol microenvironment
Henry P Oswin[SUP] 1 [/SUP], Allen E Haddrell[SUP] 1 [/SUP], Mara Otero-Fernandez[SUP] 1 [/SUP], Jamie F S Mann[SUP] 2 [/SUP], Tristan A Cogan[SUP] 2 [/SUP], Thomas G Hilditch[SUP] 1 [/SUP], Jianghan Tian[SUP] 1 [/SUP], Daniel A Hardy[SUP] 1 [/SUP], Darryl J Hill[SUP] 3 [/SUP], Adam Finn[SUP] 3 [/SUP], Andrew D Davidson[SUP] 3 [/SUP], Jonathan P Reid[SUP] 1 [/SUP]
Affiliations
Abstract
Understanding the factors that influence the airborne survival of viruses such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in aerosols is important for identifying routes of transmission and the value of various mitigation strategies for preventing transmission. We present measurements of the stability of SARS-CoV-2 in aerosol droplets (∼5 to 10 µm equilibrated radius) over timescales spanning 5 s to 20 min using an instrument to probe survival in a small population of droplets (typically 5 to 10) containing ∼1 virus/droplet. Measurements of airborne infectivity change are coupled with a detailed physicochemical analysis of the airborne droplets containing the virus. A decrease in infectivity to ∼10% of the starting value was observable for SARS-CoV-2 over 20 min, with a large proportion of the loss occurring within the first 5 min after aerosolization. The initial rate of infectivity loss was found to correlate with physical transformation of the equilibrating droplet; salts within the droplets crystallize at relative humidities (RHs) below 50%, leading to a near-instant loss of infectivity in 50 to 60% of the virus. However, at 90% RH, the droplet remains homogenous and aqueous, and the viral stability is sustained for the first 2 min, beyond which it decays to only 10% remaining infectious after 10 min. The loss of infectivity at high RH is consistent with an elevation in the pH of the droplets, caused by volatilization of CO[SUB]2[/SUB] from bicarbonate buffer within the droplet. Four different variants of SARS-CoV-2 were compared and found to have a similar degree of airborne stability at both high and low RH.
Keywords: SARS-CoV-2; aerosol; airborne transmission; environmental conditions; microphysics.
. 2022 Jul 5;119(27):e2200109119.
doi: 10.1073/pnas.2200109119. Epub 2022 Jun 28.
The dynamics of SARS-CoV-2 infectivity with changes in aerosol microenvironment
Henry P Oswin[SUP] 1 [/SUP], Allen E Haddrell[SUP] 1 [/SUP], Mara Otero-Fernandez[SUP] 1 [/SUP], Jamie F S Mann[SUP] 2 [/SUP], Tristan A Cogan[SUP] 2 [/SUP], Thomas G Hilditch[SUP] 1 [/SUP], Jianghan Tian[SUP] 1 [/SUP], Daniel A Hardy[SUP] 1 [/SUP], Darryl J Hill[SUP] 3 [/SUP], Adam Finn[SUP] 3 [/SUP], Andrew D Davidson[SUP] 3 [/SUP], Jonathan P Reid[SUP] 1 [/SUP]
Affiliations
- PMID: 35763573
- DOI: 10.1073/pnas.2200109119
Abstract
Understanding the factors that influence the airborne survival of viruses such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in aerosols is important for identifying routes of transmission and the value of various mitigation strategies for preventing transmission. We present measurements of the stability of SARS-CoV-2 in aerosol droplets (∼5 to 10 µm equilibrated radius) over timescales spanning 5 s to 20 min using an instrument to probe survival in a small population of droplets (typically 5 to 10) containing ∼1 virus/droplet. Measurements of airborne infectivity change are coupled with a detailed physicochemical analysis of the airborne droplets containing the virus. A decrease in infectivity to ∼10% of the starting value was observable for SARS-CoV-2 over 20 min, with a large proportion of the loss occurring within the first 5 min after aerosolization. The initial rate of infectivity loss was found to correlate with physical transformation of the equilibrating droplet; salts within the droplets crystallize at relative humidities (RHs) below 50%, leading to a near-instant loss of infectivity in 50 to 60% of the virus. However, at 90% RH, the droplet remains homogenous and aqueous, and the viral stability is sustained for the first 2 min, beyond which it decays to only 10% remaining infectious after 10 min. The loss of infectivity at high RH is consistent with an elevation in the pH of the droplets, caused by volatilization of CO[SUB]2[/SUB] from bicarbonate buffer within the droplet. Four different variants of SARS-CoV-2 were compared and found to have a similar degree of airborne stability at both high and low RH.
Keywords: SARS-CoV-2; aerosol; airborne transmission; environmental conditions; microphysics.
