tetano
Editor, Senior Moderator
Proc Natl Acad Sci U S A
. 2020 Jun 22;202009799.
doi: 10.1073/pnas.2009799117. Online ahead of print.
Syrian Hamsters as a Small Animal Model for SARS-CoV-2 Infection and Countermeasure Development
Masaki Imai[SUP] 1 [/SUP], Kiyoko Iwatsuki-Horimoto[SUP] 1 [/SUP], Masato Hatta[SUP] 2 [/SUP], Samantha Loeber[SUP] 3 [/SUP], Peter J Halfmann[SUP] 2 [/SUP], Noriko Nakajima[SUP] 4 [/SUP], Tokiko Watanabe[SUP] 1 [/SUP], Michiko Ujie[SUP] 1 [/SUP], Kenta Takahashi[SUP] 4 [/SUP], Mutsumi Ito[SUP] 1 [/SUP], Shinya Yamada[SUP] 1 [/SUP], Shufang Fan[SUP] 2 [/SUP], Shiho Chiba[SUP] 2 [/SUP], Makoto Kuroda[SUP] 2 [/SUP], Lizheng Guan[SUP] 2 [/SUP], Kosuke Takada[SUP] 1 [/SUP], Tammy Armbrust[SUP] 2 [/SUP], Aaron Balogh[SUP] 2 [/SUP], Yuri Furusawa[SUP] 1 [/SUP], Moe Okuda[SUP] 1 [/SUP], Hiroshi Ueki[SUP] 1 [/SUP], Atsuhiro Yasuhara[SUP] 1 [/SUP], Yuko Sakai-Tagawa[SUP] 1 [/SUP], Tiago J S Lopes[SUP] 1 2 [/SUP], Maki Kiso[SUP] 1 [/SUP], Seiya Yamayoshi[SUP] 1 [/SUP], Noriko Kinoshita[SUP] 5 [/SUP], Norio Ohmagari[SUP] 5 [/SUP], Shin-Ichiro Hattori[SUP] 5 [/SUP], Makoto Takeda[SUP] 6 [/SUP], Hiroaki Mitsuya[SUP] 5 [/SUP], Florian Krammer[SUP] 7 [/SUP], Tadaki Suzuki[SUP] 4 [/SUP], Yoshihiro Kawaoka[SUP] 8 2 9 [/SUP]
Affiliations
Abstract
At the end of 2019, a novel coronavirus (severe acute respiratory syndrome coronavirus 2; SARS-CoV-2) was detected in Wuhan, China, that spread rapidly around the world, with severe consequences for human health and the global economy. Here, we assessed the replicative ability and pathogenesis of SARS-CoV-2 isolates in Syrian hamsters. SARS-CoV-2 isolates replicated efficiently in the lungs of hamsters, causing severe pathological lung lesions following intranasal infection. In addition, microcomputed tomographic imaging revealed severe lung injury that shared characteristics with SARS-CoV-2-infected human lung, including severe, bilateral, peripherally distributed, multilobular ground glass opacity, and regions of lung consolidation. SARS-CoV-2-infected hamsters mounted neutralizing antibody responses and were protected against subsequent rechallenge with SARS-CoV-2. Moreover, passive transfer of convalescent serum to na?ve hamsters efficiently suppressed the replication of the virus in the lungs even when the serum was administrated 2 d postinfection of the serum-treated hamsters. Collectively, these findings demonstrate that this Syrian hamster model will be useful for understanding SARS-CoV-2 pathogenesis and testing vaccines and antiviral drugs.
Keywords: SARS-CoV-2; Syrian hamsters; countermeasure; infection.
. 2020 Jun 22;202009799.
doi: 10.1073/pnas.2009799117. Online ahead of print.
Syrian Hamsters as a Small Animal Model for SARS-CoV-2 Infection and Countermeasure Development
Masaki Imai[SUP] 1 [/SUP], Kiyoko Iwatsuki-Horimoto[SUP] 1 [/SUP], Masato Hatta[SUP] 2 [/SUP], Samantha Loeber[SUP] 3 [/SUP], Peter J Halfmann[SUP] 2 [/SUP], Noriko Nakajima[SUP] 4 [/SUP], Tokiko Watanabe[SUP] 1 [/SUP], Michiko Ujie[SUP] 1 [/SUP], Kenta Takahashi[SUP] 4 [/SUP], Mutsumi Ito[SUP] 1 [/SUP], Shinya Yamada[SUP] 1 [/SUP], Shufang Fan[SUP] 2 [/SUP], Shiho Chiba[SUP] 2 [/SUP], Makoto Kuroda[SUP] 2 [/SUP], Lizheng Guan[SUP] 2 [/SUP], Kosuke Takada[SUP] 1 [/SUP], Tammy Armbrust[SUP] 2 [/SUP], Aaron Balogh[SUP] 2 [/SUP], Yuri Furusawa[SUP] 1 [/SUP], Moe Okuda[SUP] 1 [/SUP], Hiroshi Ueki[SUP] 1 [/SUP], Atsuhiro Yasuhara[SUP] 1 [/SUP], Yuko Sakai-Tagawa[SUP] 1 [/SUP], Tiago J S Lopes[SUP] 1 2 [/SUP], Maki Kiso[SUP] 1 [/SUP], Seiya Yamayoshi[SUP] 1 [/SUP], Noriko Kinoshita[SUP] 5 [/SUP], Norio Ohmagari[SUP] 5 [/SUP], Shin-Ichiro Hattori[SUP] 5 [/SUP], Makoto Takeda[SUP] 6 [/SUP], Hiroaki Mitsuya[SUP] 5 [/SUP], Florian Krammer[SUP] 7 [/SUP], Tadaki Suzuki[SUP] 4 [/SUP], Yoshihiro Kawaoka[SUP] 8 2 9 [/SUP]
Affiliations
- PMID: 32571934
- DOI: 10.1073/pnas.2009799117
Abstract
At the end of 2019, a novel coronavirus (severe acute respiratory syndrome coronavirus 2; SARS-CoV-2) was detected in Wuhan, China, that spread rapidly around the world, with severe consequences for human health and the global economy. Here, we assessed the replicative ability and pathogenesis of SARS-CoV-2 isolates in Syrian hamsters. SARS-CoV-2 isolates replicated efficiently in the lungs of hamsters, causing severe pathological lung lesions following intranasal infection. In addition, microcomputed tomographic imaging revealed severe lung injury that shared characteristics with SARS-CoV-2-infected human lung, including severe, bilateral, peripherally distributed, multilobular ground glass opacity, and regions of lung consolidation. SARS-CoV-2-infected hamsters mounted neutralizing antibody responses and were protected against subsequent rechallenge with SARS-CoV-2. Moreover, passive transfer of convalescent serum to na?ve hamsters efficiently suppressed the replication of the virus in the lungs even when the serum was administrated 2 d postinfection of the serum-treated hamsters. Collectively, these findings demonstrate that this Syrian hamster model will be useful for understanding SARS-CoV-2 pathogenesis and testing vaccines and antiviral drugs.
Keywords: SARS-CoV-2; Syrian hamsters; countermeasure; infection.