tetano
Editor, Senior Moderator
Proc Natl Acad Sci U S A
. 2023 Mar 14;120(11):e2220272120.
doi: 10.1073/pnas.2220272120. Epub 2023 Mar 7.
Nasal administration of anti-CD3 mAb (Foralumab) downregulates NKG7 and increases TGFB1 and GIMAP7 expression in T cells in subjects with COVID-19
Thais G Moreira[SUP] 1 [/SUP], Christian D Gauthier[SUP] 1 [/SUP], Liam Murphy[SUP] 1 [/SUP], Toby B Lanser[SUP] 1 [/SUP], Anu Paul[SUP] 1 [/SUP], Kimble T F Matos[SUP] 2 [/SUP], Davide Mangani[SUP] 1 [/SUP], Saef Izzy[SUP] 1 [/SUP], Rafael M Rezende[SUP] 1 [/SUP], Brian C Healy[SUP] 1 [/SUP], Clare M Baecher-Allan[SUP] 1 [/SUP], Tanuja Chitnis[SUP] 1 [/SUP], Vijay Kuchroo[SUP] 1 [/SUP], Howard L Weiner[SUP] 1 [/SUP]
Affiliations
Abstract
T cells are present in early stages of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and play a major role in disease outcome and long-lasting immunity. Nasal administration of a fully human anti-CD3 monoclonal antibody (Foralumab) reduced lung inflammation as well as serum IL-6 and C-reactive protein in moderate cases of COVID-19. Using serum proteomics and RNA-sequencing, we investigated the immune changes in patients treated with nasal Foralumab. In a randomized trial, mild to moderate COVID-19 outpatients received nasal Foralumab (100 μg/d) given for 10 consecutive days and were compared to patients that did not receive Foralumab. We found that naïve-like T cells were increased in Foralumab-treated subjects and NGK7[SUP]+[/SUP] effector T cells were reduced. CCL5, IL32, CST7, GZMH, GZMB, GZMA, PRF1, and CCL4 gene expression were downregulated in T cells and CASP1 was downregulated in T cells, monocytes, and B cells in subjects treated with Foralumab. In addition to the downregulation of effector features, an increase in TGFB1 gene expression in cell types with known effector function was observed in Foralumab-treated subjects. We also found increased expression of GTP-binding gene GIMAP7 in subjects treated with Foralumab. Rho/ROCK1, a downstream pathway of GTPases signaling was downregulated in Foralumab-treated individuals. TGFB1, GIMAP7, and NKG7 transcriptomic changes observed in Foralumab-treated COVID-19 subjects were also observed in healthy volunteers, MS subjects, and mice treated with nasal anti-CD3. Our findings demonstrate that nasal Foralumab modulates the inflammatory response in COVID-19 and provides a novel avenue to treat the disease.
Keywords: COVID-19; Foralumab; SARS-CoV-2; T cells; anti-CD3.
. 2023 Mar 14;120(11):e2220272120.
doi: 10.1073/pnas.2220272120. Epub 2023 Mar 7.
Nasal administration of anti-CD3 mAb (Foralumab) downregulates NKG7 and increases TGFB1 and GIMAP7 expression in T cells in subjects with COVID-19
Thais G Moreira[SUP] 1 [/SUP], Christian D Gauthier[SUP] 1 [/SUP], Liam Murphy[SUP] 1 [/SUP], Toby B Lanser[SUP] 1 [/SUP], Anu Paul[SUP] 1 [/SUP], Kimble T F Matos[SUP] 2 [/SUP], Davide Mangani[SUP] 1 [/SUP], Saef Izzy[SUP] 1 [/SUP], Rafael M Rezende[SUP] 1 [/SUP], Brian C Healy[SUP] 1 [/SUP], Clare M Baecher-Allan[SUP] 1 [/SUP], Tanuja Chitnis[SUP] 1 [/SUP], Vijay Kuchroo[SUP] 1 [/SUP], Howard L Weiner[SUP] 1 [/SUP]
Affiliations
- PMID: 36881624
- DOI: 10.1073/pnas.2220272120
Abstract
T cells are present in early stages of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and play a major role in disease outcome and long-lasting immunity. Nasal administration of a fully human anti-CD3 monoclonal antibody (Foralumab) reduced lung inflammation as well as serum IL-6 and C-reactive protein in moderate cases of COVID-19. Using serum proteomics and RNA-sequencing, we investigated the immune changes in patients treated with nasal Foralumab. In a randomized trial, mild to moderate COVID-19 outpatients received nasal Foralumab (100 μg/d) given for 10 consecutive days and were compared to patients that did not receive Foralumab. We found that naïve-like T cells were increased in Foralumab-treated subjects and NGK7[SUP]+[/SUP] effector T cells were reduced. CCL5, IL32, CST7, GZMH, GZMB, GZMA, PRF1, and CCL4 gene expression were downregulated in T cells and CASP1 was downregulated in T cells, monocytes, and B cells in subjects treated with Foralumab. In addition to the downregulation of effector features, an increase in TGFB1 gene expression in cell types with known effector function was observed in Foralumab-treated subjects. We also found increased expression of GTP-binding gene GIMAP7 in subjects treated with Foralumab. Rho/ROCK1, a downstream pathway of GTPases signaling was downregulated in Foralumab-treated individuals. TGFB1, GIMAP7, and NKG7 transcriptomic changes observed in Foralumab-treated COVID-19 subjects were also observed in healthy volunteers, MS subjects, and mice treated with nasal anti-CD3. Our findings demonstrate that nasal Foralumab modulates the inflammatory response in COVID-19 and provides a novel avenue to treat the disease.
Keywords: COVID-19; Foralumab; SARS-CoV-2; T cells; anti-CD3.