tetano
Editor, Senior Moderator
Proc Natl Acad Sci U S A
. 2024 May 28;121(22):e2314166121.
doi: 10.1073/pnas.2314166121. Epub 2024 May 20. Inhibition of mRNA nuclear export promotes SARS-CoV-2 pathogenesis
Menghan Mei[SUP] #[/SUP][SUP] 1 [/SUP], Anastasija Cupic[SUP] #[/SUP][SUP] 2 3 [/SUP], Lisa Miorin[SUP] 2 4 [/SUP], Chengjin Ye[SUP] 5 [/SUP], Tolga Cagatay[SUP] 6 [/SUP], Ke Zhang[SUP] 6 7 [/SUP], Komal Patel[SUP] 1 8 [/SUP], Natalie Wilson[SUP] 1 [/SUP], W Hayes McDonald[SUP] 1 9 [/SUP], Nicholas A Crossland[SUP] 10 11 [/SUP], Ming Lo[SUP] 10 [/SUP], Magdalena Rutkowska[SUP] 2 3 [/SUP], Sadaf Aslam[SUP] 2 [/SUP], Ignacio Mena[SUP] 2 4 [/SUP], Luis Martinez-Sobrido[SUP] 5 [/SUP], Yi Ren[SUP] 1 [/SUP], Adolfo García-Sastre[SUP] 2 4 12 13 14 [/SUP], Beatriz M A Fontoura[SUP] 6 [/SUP]
Affiliations
The nonstructural protein 1 (Nsp1) of SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) is a virulence factor that targets multiple cellular pathways to inhibit host gene expression and antiviral response. However, the underlying mechanisms of the various Nsp1-mediated functions and their contributions to SARS-CoV-2 virulence remain unclear. Among the targets of Nsp1 is the mRNA (messenger ribonucleic acid) export receptor NXF1-NXT1, which mediates nuclear export of mRNAs from the nucleus to the cytoplasm. Based on Nsp1 crystal structure, we generated mutants on Nsp1 surfaces and identified an acidic N-terminal patch that is critical for interaction with NXF1-NXT1. Photoactivatable Nsp1 probe reveals the RNA Recognition Motif (RRM) domain of NXF1 as an Nsp1 N-terminal binding site. By mutating the Nsp1 N-terminal acidic patch, we identified a separation-of-function mutant of Nsp1 that retains its translation inhibitory function but substantially loses its interaction with NXF1 and reverts Nsp1-mediated mRNA export inhibition. We then generated a recombinant (r)SARS-CoV-2 mutant on the Nsp1 N-terminal acidic patch and found that this surface is key to promote NXF1 binding and inhibition of host mRNA nuclear export, viral replication, and pathogenicity in vivo. Thus, these findings provide a mechanistic understanding of Nsp1-mediated mRNA export inhibition and establish the importance of this pathway in the virulence of SARS-CoV-2.
Keywords: NXF1; Nsp1; SARS-CoV-2; mRNA export; nuclear transport.
. 2024 May 28;121(22):e2314166121.
doi: 10.1073/pnas.2314166121. Epub 2024 May 20. Inhibition of mRNA nuclear export promotes SARS-CoV-2 pathogenesis
Menghan Mei[SUP] #[/SUP][SUP] 1 [/SUP], Anastasija Cupic[SUP] #[/SUP][SUP] 2 3 [/SUP], Lisa Miorin[SUP] 2 4 [/SUP], Chengjin Ye[SUP] 5 [/SUP], Tolga Cagatay[SUP] 6 [/SUP], Ke Zhang[SUP] 6 7 [/SUP], Komal Patel[SUP] 1 8 [/SUP], Natalie Wilson[SUP] 1 [/SUP], W Hayes McDonald[SUP] 1 9 [/SUP], Nicholas A Crossland[SUP] 10 11 [/SUP], Ming Lo[SUP] 10 [/SUP], Magdalena Rutkowska[SUP] 2 3 [/SUP], Sadaf Aslam[SUP] 2 [/SUP], Ignacio Mena[SUP] 2 4 [/SUP], Luis Martinez-Sobrido[SUP] 5 [/SUP], Yi Ren[SUP] 1 [/SUP], Adolfo García-Sastre[SUP] 2 4 12 13 14 [/SUP], Beatriz M A Fontoura[SUP] 6 [/SUP]
Affiliations
- PMID: 38768348
- DOI: 10.1073/pnas.2314166121
The nonstructural protein 1 (Nsp1) of SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) is a virulence factor that targets multiple cellular pathways to inhibit host gene expression and antiviral response. However, the underlying mechanisms of the various Nsp1-mediated functions and their contributions to SARS-CoV-2 virulence remain unclear. Among the targets of Nsp1 is the mRNA (messenger ribonucleic acid) export receptor NXF1-NXT1, which mediates nuclear export of mRNAs from the nucleus to the cytoplasm. Based on Nsp1 crystal structure, we generated mutants on Nsp1 surfaces and identified an acidic N-terminal patch that is critical for interaction with NXF1-NXT1. Photoactivatable Nsp1 probe reveals the RNA Recognition Motif (RRM) domain of NXF1 as an Nsp1 N-terminal binding site. By mutating the Nsp1 N-terminal acidic patch, we identified a separation-of-function mutant of Nsp1 that retains its translation inhibitory function but substantially loses its interaction with NXF1 and reverts Nsp1-mediated mRNA export inhibition. We then generated a recombinant (r)SARS-CoV-2 mutant on the Nsp1 N-terminal acidic patch and found that this surface is key to promote NXF1 binding and inhibition of host mRNA nuclear export, viral replication, and pathogenicity in vivo. Thus, these findings provide a mechanistic understanding of Nsp1-mediated mRNA export inhibition and establish the importance of this pathway in the virulence of SARS-CoV-2.
Keywords: NXF1; Nsp1; SARS-CoV-2; mRNA export; nuclear transport.