tetano
Editor, Senior Moderator
Proc Natl Acad Sci U S A
. 2020 Jul 28;202006824.
doi: 10.1073/pnas.2006824117. Online ahead of print.
Haplotype networks of SARS-CoV-2 infections in the Diamond Princess cruise ship outbreak
Tsuyoshi Sekizuka[SUP] 1 [/SUP], Kentaro Itokawa[SUP] 1 [/SUP], Tsutomu Kageyama[SUP] 2 [/SUP], Shinji Saito[SUP] 2 [/SUP], Ikuyo Takayama[SUP] 2 [/SUP], Hideki Asanuma[SUP] 2 [/SUP], Naganori Nao[SUP] 3 [/SUP], Rina Tanaka[SUP] 1 [/SUP], Masanori Hashino[SUP] 1 [/SUP], Takuri Takahashi[SUP] 4 [/SUP], Hajime Kamiya[SUP] 4 [/SUP], Takuya Yamagishi[SUP] 4 [/SUP], Kensaku Kakimoto[SUP] 4 [/SUP], Motoi Suzuki[SUP] 4 [/SUP], Hideki Hasegawa[SUP] 2 [/SUP], Takaji Wakita[SUP] 5 [/SUP], Makoto Kuroda[SUP] 6 [/SUP]
Affiliations
Abstract
The Diamond Princess cruise ship was put under quarantine offshore Yokohama, Japan, after a passenger who disembarked in Hong Kong was confirmed as a coronavirus disease 2019 case. We performed whole-genome sequencing of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) directly from PCR[SUP]+[/SUP] clinical specimens and conducted a phylogenetic analysis of the outbreak. All tested isolates exhibited a transversion at G[SUB]11083[/SUB]T, suggesting that SARS-CoV-2 dissemination on the Diamond Princess originated from a single introduction event before the quarantine started. Although further spreading might have been prevented by quarantine, some progeny clusters could be linked to transmission through mass-gathering events in the recreational areas and direct transmission among passengers who shared cabins during the quarantine. This study demonstrates the usefulness of haplotype network/phylogeny analysis in identifying potential infection routes.
Keywords: COVID-19; Diamond Princess; SARS-CoV-2; haplotypes.
. 2020 Jul 28;202006824.
doi: 10.1073/pnas.2006824117. Online ahead of print.
Haplotype networks of SARS-CoV-2 infections in the Diamond Princess cruise ship outbreak
Tsuyoshi Sekizuka[SUP] 1 [/SUP], Kentaro Itokawa[SUP] 1 [/SUP], Tsutomu Kageyama[SUP] 2 [/SUP], Shinji Saito[SUP] 2 [/SUP], Ikuyo Takayama[SUP] 2 [/SUP], Hideki Asanuma[SUP] 2 [/SUP], Naganori Nao[SUP] 3 [/SUP], Rina Tanaka[SUP] 1 [/SUP], Masanori Hashino[SUP] 1 [/SUP], Takuri Takahashi[SUP] 4 [/SUP], Hajime Kamiya[SUP] 4 [/SUP], Takuya Yamagishi[SUP] 4 [/SUP], Kensaku Kakimoto[SUP] 4 [/SUP], Motoi Suzuki[SUP] 4 [/SUP], Hideki Hasegawa[SUP] 2 [/SUP], Takaji Wakita[SUP] 5 [/SUP], Makoto Kuroda[SUP] 6 [/SUP]
Affiliations
- PMID: 32723824
- DOI: 10.1073/pnas.2006824117
Abstract
The Diamond Princess cruise ship was put under quarantine offshore Yokohama, Japan, after a passenger who disembarked in Hong Kong was confirmed as a coronavirus disease 2019 case. We performed whole-genome sequencing of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) directly from PCR[SUP]+[/SUP] clinical specimens and conducted a phylogenetic analysis of the outbreak. All tested isolates exhibited a transversion at G[SUB]11083[/SUB]T, suggesting that SARS-CoV-2 dissemination on the Diamond Princess originated from a single introduction event before the quarantine started. Although further spreading might have been prevented by quarantine, some progeny clusters could be linked to transmission through mass-gathering events in the recreational areas and direct transmission among passengers who shared cabins during the quarantine. This study demonstrates the usefulness of haplotype network/phylogeny analysis in identifying potential infection routes.
Keywords: COVID-19; Diamond Princess; SARS-CoV-2; haplotypes.