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Proc Natl Acad Sci U S A. Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells

tetano

Editor, Senior Moderator
Proc Natl Acad Sci U S A. 2020 Mar 12. pii: 202002589. doi: 10.1073/pnas.2002589117. [Epub ahead of print]
Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells.


Matsuyama S[SUP]1[/SUP], Nao N[SUP]2[/SUP], Shirato K[SUP]2[/SUP], Kawase M[SUP]2[/SUP], Saito S[SUP]3[/SUP], Takayama I[SUP]3[/SUP], Nagata N[SUP]4[/SUP], Sekizuka T[SUP]5[/SUP], Katoh H[SUP]2[/SUP], Kato F[SUP]2[/SUP], Sakata M[SUP]2[/SUP], Tahara M[SUP]2[/SUP], Kutsuna S[SUP]6[/SUP], Ohmagari N[SUP]6[/SUP], Kuroda M[SUP]5[/SUP], Suzuki T[SUP]4[/SUP], Kageyama T[SUP]3[/SUP], Takeda M[SUP]1[/SUP].

Author information




Abstract

A novel betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused a large respiratory outbreak in Wuhan, China in December 2019, is currently spreading across many countries globally. Here, we show that a TMPRSS2-expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it useful for isolating and propagating SARS-CoV-2. Our results reveal that, in common with SARS- and Middle East respiratory syndrome-CoV, SARS-CoV-2 infection is enhanced by TMPRSS2.
Copyright ? 2020 the Author(s). Published by PNAS.



KEYWORDS:

SARS-CoV-2; TMPRSS2; VeroE6; coronavirus; outbreak


PMID:32165541DOI:10.1073/pnas.2002589117
 
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