tetano
Editor, Senior Moderator
Food Chem Toxicol. 2019 Nov 22:110985. doi: 10.1016/j.fct.2019.110985. [Epub ahead of print] [h=1]Probing the effect of quercetin 3-glucoside from Dianthus superbus L against influenza virus infection- In vitro and in silico biochemical and toxicological screening.[/h]
Nile SH[SUP]1[/SUP], Kim DH[SUP]2[/SUP], Nile A[SUP]2[/SUP], Park GS[SUP]2[/SUP], Gansukh E[SUP]3[/SUP], Kai G[SUP]4[/SUP].
[h=3]Author information[/h] 1 Laboratory of Medicinal Plant Biotechnology, College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, PR China. 2 Department of Bio-resources and Food Science, Konkuk University, Seoul, 143701, South Korea. 3 Department of Bio-resources and Food Science, Konkuk University, Seoul, 143701, South Korea; Department of Life Science and Biotechnology, Huree University, Ulaanbaatar, Mongolia. Electronic address: enkhtaivan11@naver.com. 4 Laboratory of Medicinal Plant Biotechnology, College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, PR China. Electronic address: guoyinkai1@126.com.
[h=3]Abstract[/h] Investigation of antiviral and cytotoxic effect of quercetin 3-glucoside (Q3G) from Dianthus superbus L over influenza virus infection and replication were studied. Moreover, anti-influenza mechanism was screened by time-dependent antiviral assay, virus-induced symptoms and related gene expressions. The blockade of cap-binding domain of polymerase basic protein subunit were analysed by molecular docking study. The Q3G demonstrated potent antiviral activity showing 4.93, 6.43, 9.94, 8.3, and 7.1 μg/mL of IC[SUB]50[/SUB] for A/PR/8/34, A/Victoria/3/75, A/WS/33, B/Maryland/1/59, and B/Lee/40, respectively. The cellular toxicity of Q3G and oseltamivir (control) were tested and >100 μg/mL of CC[SUB]50[/SUB] value considered as nontoxic. Influenza A virus infection induced a higher ROS production, however potentially reduced by Q3G treatment and significantly blocked virus infection induced acidic vesicular organelles (AVO). Moreover, Q3G has no inhibitory effect for neuraminidase activity but blocked virus replication through time dependent assay and showed more competitive binding affinity (-8.0 kcal/mal) than GTP (-7.0 kcal/mol) to block polymerase basic protein-2 subunit of influenza virus. Q3G from D. superbus showed potent antiviral activity against influenza A and B viruses with suppressive effect on virus-induced cellular ROS generation and AVO formation. Thus, this study provided a new line of research for Q3G to develop possible natural anti-influenza drug.
Copyright ? 2019. Published by Elsevier Ltd.
[h=4]KEYWORDS:[/h] Antiviral; Dianthus superbus; Influenza virus; Polymerase basic protein; Quercetin 3-glucoside
PMID: 31765700 DOI: 10.1016/j.fct.2019.110985
Nile SH[SUP]1[/SUP], Kim DH[SUP]2[/SUP], Nile A[SUP]2[/SUP], Park GS[SUP]2[/SUP], Gansukh E[SUP]3[/SUP], Kai G[SUP]4[/SUP].
[h=3]Author information[/h] 1 Laboratory of Medicinal Plant Biotechnology, College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, PR China. 2 Department of Bio-resources and Food Science, Konkuk University, Seoul, 143701, South Korea. 3 Department of Bio-resources and Food Science, Konkuk University, Seoul, 143701, South Korea; Department of Life Science and Biotechnology, Huree University, Ulaanbaatar, Mongolia. Electronic address: enkhtaivan11@naver.com. 4 Laboratory of Medicinal Plant Biotechnology, College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, PR China. Electronic address: guoyinkai1@126.com.
[h=3]Abstract[/h] Investigation of antiviral and cytotoxic effect of quercetin 3-glucoside (Q3G) from Dianthus superbus L over influenza virus infection and replication were studied. Moreover, anti-influenza mechanism was screened by time-dependent antiviral assay, virus-induced symptoms and related gene expressions. The blockade of cap-binding domain of polymerase basic protein subunit were analysed by molecular docking study. The Q3G demonstrated potent antiviral activity showing 4.93, 6.43, 9.94, 8.3, and 7.1 μg/mL of IC[SUB]50[/SUB] for A/PR/8/34, A/Victoria/3/75, A/WS/33, B/Maryland/1/59, and B/Lee/40, respectively. The cellular toxicity of Q3G and oseltamivir (control) were tested and >100 μg/mL of CC[SUB]50[/SUB] value considered as nontoxic. Influenza A virus infection induced a higher ROS production, however potentially reduced by Q3G treatment and significantly blocked virus infection induced acidic vesicular organelles (AVO). Moreover, Q3G has no inhibitory effect for neuraminidase activity but blocked virus replication through time dependent assay and showed more competitive binding affinity (-8.0 kcal/mal) than GTP (-7.0 kcal/mol) to block polymerase basic protein-2 subunit of influenza virus. Q3G from D. superbus showed potent antiviral activity against influenza A and B viruses with suppressive effect on virus-induced cellular ROS generation and AVO formation. Thus, this study provided a new line of research for Q3G to develop possible natural anti-influenza drug.
Copyright ? 2019. Published by Elsevier Ltd.
[h=4]KEYWORDS:[/h] Antiviral; Dianthus superbus; Influenza virus; Polymerase basic protein; Quercetin 3-glucoside
PMID: 31765700 DOI: 10.1016/j.fct.2019.110985