Ganseerpel
Advisory Board, Senior Moderator
Virus-Specific CD8+ T Cells in the Liver: Armed and Ready to Kill.
J Immunol. 2007 Mar 1;178(5):2737-2745.
Keating R, Yue W, Rutigliano JA, So J, Olivas E, Thomas PG, Doherty PC.
Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 38105.
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?tmpl=NoSidebarfile&db=PubMed&cmd=Retrieve&list_uids=17312116&dopt=Abstract<o
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Influenza A virus infection of C57BL/6 mice is a well-characterized model for studying CD8(+) T cell-mediated immunity. Analysis of primary and secondary responses showed that the liver is highly enriched for CD8(+) T cells specific for the immunodominant H2D(b)NP(366-374) (D(b)NP(366)) epitope. Functional analysis established that these liver-derived virus-specific CD8(+) T cells are fully competent cytotoxic effectors and IFN-gamma secretors. In addition, flow cytometric analysis of early apoptotic cells showed that these influenza-specific CD8(+) T cells from liver are as viable as those in the spleen, bronchoalveolar lavage, mediastinal lymph nodes, or lung. Moreover, cytokine profiles of the influenza-specific CD8(+) T cells recovered from different sites were consistent with the bronchoalveolar lavage, rather than liver population, being the most susceptible to activation-induced cell death. Importantly, adoptively transferred influenza virus-specific CD8(+) T cells from the liver survived and were readily recalled after virus challenge. Together, these results show clearly that the liver is not a "graveyard" for influenza virus-specific CD8(+) T cells.
PMID: 17312116 [PubMed - as supplied by publisher]<o></o>
J Immunol. 2007 Mar 1;178(5):2737-2745.
Keating R, Yue W, Rutigliano JA, So J, Olivas E, Thomas PG, Doherty PC.
Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 38105.
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?tmpl=NoSidebarfile&db=PubMed&cmd=Retrieve&list_uids=17312116&dopt=Abstract<o
></o>Influenza A virus infection of C57BL/6 mice is a well-characterized model for studying CD8(+) T cell-mediated immunity. Analysis of primary and secondary responses showed that the liver is highly enriched for CD8(+) T cells specific for the immunodominant H2D(b)NP(366-374) (D(b)NP(366)) epitope. Functional analysis established that these liver-derived virus-specific CD8(+) T cells are fully competent cytotoxic effectors and IFN-gamma secretors. In addition, flow cytometric analysis of early apoptotic cells showed that these influenza-specific CD8(+) T cells from liver are as viable as those in the spleen, bronchoalveolar lavage, mediastinal lymph nodes, or lung. Moreover, cytokine profiles of the influenza-specific CD8(+) T cells recovered from different sites were consistent with the bronchoalveolar lavage, rather than liver population, being the most susceptible to activation-induced cell death. Importantly, adoptively transferred influenza virus-specific CD8(+) T cells from the liver survived and were readily recalled after virus challenge. Together, these results show clearly that the liver is not a "graveyard" for influenza virus-specific CD8(+) T cells.
PMID: 17312116 [PubMed - as supplied by publisher]<o
></o>