tetano
Editor, Senior Moderator
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2012 Feb;28(2):177-80.
[Preparation and partial characterization of monoclonal antibodies against HA protein of H1 subtype influenza virus].
[Article in Chinese]
Guo CY, Li HJ, Liu Y, Zhao XR, Wang X, Feng Q, Li Y, Hu J.
Source
Shaanxi Provincial People's Hospital, the Third Affiliated Hospital, Xi'an Jiaotong University College of Medicine, Xi'an 710068, China.
Abstract
AIM:
Preparation of H1 subtype of influenza virus HA protein monoclonal antibody (mAbs), and to analyse their reactivity.
METHODS:
H1N1 influenza virus vaccine (2009) and seasonal A1 influenza virus vaccine as antigen, conventional immunization, fusion, cloning, access to the antigen-specific mAbs. To study the reactivity and specificity of mAbs by ELISA, HI test and Western blot.
RESULTS:
We have obtained 97 hybridoma of stable secreting anti-H1 subtype of influenza virus HA protein. According to their different reactivity, these mAbs can be divided into four categories: 39 strains for strain-specific, of which 29 have HI activity; 7 strains for subtype-specific, of which 5 have HI activity; 16 strains for the 2009 pandemic strain and the seasonal strains to common antigens, of which 9 have HI activity; 35 strains common for influenza virus antigens, of which 22 have HI activity.
CONCLUSION:
Both vaccines have better immunogenicity and immune protection activity. Preparation of four types antibody against HA protein of H1 subtype of influenza virus, can provide experimental data for preparation subtype-specific diagnostic kits and influenza A and B virus diagnostic kit, and lay a foundation for further studying of H1N1 influenza virus HA epitope.
PMID:
22304780
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/22304780
[Preparation and partial characterization of monoclonal antibodies against HA protein of H1 subtype influenza virus].
[Article in Chinese]
Guo CY, Li HJ, Liu Y, Zhao XR, Wang X, Feng Q, Li Y, Hu J.
Source
Shaanxi Provincial People's Hospital, the Third Affiliated Hospital, Xi'an Jiaotong University College of Medicine, Xi'an 710068, China.
Abstract
AIM:
Preparation of H1 subtype of influenza virus HA protein monoclonal antibody (mAbs), and to analyse their reactivity.
METHODS:
H1N1 influenza virus vaccine (2009) and seasonal A1 influenza virus vaccine as antigen, conventional immunization, fusion, cloning, access to the antigen-specific mAbs. To study the reactivity and specificity of mAbs by ELISA, HI test and Western blot.
RESULTS:
We have obtained 97 hybridoma of stable secreting anti-H1 subtype of influenza virus HA protein. According to their different reactivity, these mAbs can be divided into four categories: 39 strains for strain-specific, of which 29 have HI activity; 7 strains for subtype-specific, of which 5 have HI activity; 16 strains for the 2009 pandemic strain and the seasonal strains to common antigens, of which 9 have HI activity; 35 strains common for influenza virus antigens, of which 22 have HI activity.
CONCLUSION:
Both vaccines have better immunogenicity and immune protection activity. Preparation of four types antibody against HA protein of H1 subtype of influenza virus, can provide experimental data for preparation subtype-specific diagnostic kits and influenza A and B virus diagnostic kit, and lay a foundation for further studying of H1N1 influenza virus HA epitope.
PMID:
22304780
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/22304780