Mary Wilson
Well-known member
December 22, 2022, 2:23pm
Daniel M Maloney1,2*; Goncalo Fernandes1*; Rebecca Dewar1; Aine O’Toole2; Chenge Mphanga3; Thomas C Williams3; Martin McHugh1; Andrew Rambaut2; Kate E Templeton1
Introduction:
Here we introduce two novel amplicon-based primer schemes for the sequencing of respiratory syncytial virus (RSV) A and RSV B genomes. With the atypical seasonality of RSV since the onset of the SARS-CoV-2 pandemic, as well as the approaching start of immunisation against RSV, there has never been as much of a demand for high quality, easy to produce genomes. The SARS-CoV-2 sequencing effort has highlighted the utility and flexibility of short amplicon based sequencing approaches, such as those designed by the ARTIC network, to enable genomic surveillance at an unprecedented scale. As such, the two primer schemes presented here have been specifically designed to slot into existing SARS-CoV-2 sequencing infrastructure to enable the easy switching of methodologies for the now extensive network of viral genomic sequencing laboratories to high throughput RSV genomic surveillance. These preliminary data presented here demonstrate the ability of these amplicon-based primer schemes to produce genomes with an average completeness of >95% from samples at least up to Ct 30, for both RSV A and RSV B.
https://virological.org/t/prelimina...sequencing-approaches-for-rsv-a-and-rsv-b/918
Daniel M Maloney1,2*; Goncalo Fernandes1*; Rebecca Dewar1; Aine O’Toole2; Chenge Mphanga3; Thomas C Williams3; Martin McHugh1; Andrew Rambaut2; Kate E Templeton1
Introduction:
Here we introduce two novel amplicon-based primer schemes for the sequencing of respiratory syncytial virus (RSV) A and RSV B genomes. With the atypical seasonality of RSV since the onset of the SARS-CoV-2 pandemic, as well as the approaching start of immunisation against RSV, there has never been as much of a demand for high quality, easy to produce genomes. The SARS-CoV-2 sequencing effort has highlighted the utility and flexibility of short amplicon based sequencing approaches, such as those designed by the ARTIC network, to enable genomic surveillance at an unprecedented scale. As such, the two primer schemes presented here have been specifically designed to slot into existing SARS-CoV-2 sequencing infrastructure to enable the easy switching of methodologies for the now extensive network of viral genomic sequencing laboratories to high throughput RSV genomic surveillance. These preliminary data presented here demonstrate the ability of these amplicon-based primer schemes to produce genomes with an average completeness of >95% from samples at least up to Ct 30, for both RSV A and RSV B.
https://virological.org/t/prelimina...sequencing-approaches-for-rsv-a-and-rsv-b/918