tetano
Editor, Senior Moderator
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2013 Oct;30(5):968-71.
[Preliminary establishment of an electrochemiluminescence immunoassay method for detection of influenza virus B].
[Article in Chinese]
Chen R, Wang J, Liu Z.
Author information
Abstract
To establish an electrochemiluminescence immunoassay (ECLIA) for the detection of influenza virus B (Flu B), we biotinylated the Flu B monoclonal antibody (mAb),labeled the paired Ab with ruthenium and used the streptavidin-coated microparticles in this study. The ECL intensity was linear with the concentrations of antigen of Flu B in the range of 0.55 microg/mL to 17.5 microg/mL, with a detection limit of 0.55 microg/mL (S/N= 3). The precision,sensitivity and specificity were evaluated. The established ECLIA for Flu B antigen in this study was specific, sensitive and precise. It may provide a convenient and rapid technique for clinical detection of Flu B.
PMID:
24459953
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/24459953
[Preliminary establishment of an electrochemiluminescence immunoassay method for detection of influenza virus B].
[Article in Chinese]
Chen R, Wang J, Liu Z.
Author information
Abstract
To establish an electrochemiluminescence immunoassay (ECLIA) for the detection of influenza virus B (Flu B), we biotinylated the Flu B monoclonal antibody (mAb),labeled the paired Ab with ruthenium and used the streptavidin-coated microparticles in this study. The ECL intensity was linear with the concentrations of antigen of Flu B in the range of 0.55 microg/mL to 17.5 microg/mL, with a detection limit of 0.55 microg/mL (S/N= 3). The precision,sensitivity and specificity were evaluated. The established ECLIA for Flu B antigen in this study was specific, sensitive and precise. It may provide a convenient and rapid technique for clinical detection of Flu B.
PMID:
24459953
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/24459953