tetano
Editor, Senior Moderator
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2013 Dec;29(12):1319-21.
[Prediction of the B cell epitopes of hemagglutinin and neuraminidase of human infected avian influenza A H7N9 virus].
[Article in Chinese]
Li H, Hu P.
Source
Department of Infectious Diseases, Second Affiliated Hospital, Chongqing Medical University, Ministry of Education Key Laboratory of Molecular Biology for Infectious Diseases, Institute of Viral Heptitis, Chongqing 400010, China.
Abstract
Objective To predict the B cell epitopes of hemagglutinin (HA) and neuraminidase (NA) of human-infected avian influenza A H7N9 virus by bioinformatics software. Methods The B cell epitopes of HA and NA proteins were predicted comprehensively by bioinformatics software such as Protean, PORTER, MEGA based on the amino acid sequences of HA and NA, and further screened by Wu Yuzhang's average antigen index strategies. Results The most possible epitopes of HA were located on its N-terminal No. 53-57, 179-183. The N-terminal No. 62-66, 98-101, 164-166, 315-320, 424-426, 443-445, 493-496 might have the B cell epitopes of HA protein. The most possible epitopes of NA were located on its N-terminal No. 321-329, 379-386. There were possible B cell epitopes located on the N-terminal No. 46-48, 52-54, 74-78, 136-141, 145-150, 160-164, 194-197, 246-248, 333-343, 426-431. Conclusion The prediction of B cell epitopes of HA and NA proteins with multiple methods benefits the research and development of B cell epitope vaccine against human infection with avian influenza A H7N9 virus.
PMID:
24321078
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/24321078
[Prediction of the B cell epitopes of hemagglutinin and neuraminidase of human infected avian influenza A H7N9 virus].
[Article in Chinese]
Li H, Hu P.
Source
Department of Infectious Diseases, Second Affiliated Hospital, Chongqing Medical University, Ministry of Education Key Laboratory of Molecular Biology for Infectious Diseases, Institute of Viral Heptitis, Chongqing 400010, China.
Abstract
Objective To predict the B cell epitopes of hemagglutinin (HA) and neuraminidase (NA) of human-infected avian influenza A H7N9 virus by bioinformatics software. Methods The B cell epitopes of HA and NA proteins were predicted comprehensively by bioinformatics software such as Protean, PORTER, MEGA based on the amino acid sequences of HA and NA, and further screened by Wu Yuzhang's average antigen index strategies. Results The most possible epitopes of HA were located on its N-terminal No. 53-57, 179-183. The N-terminal No. 62-66, 98-101, 164-166, 315-320, 424-426, 443-445, 493-496 might have the B cell epitopes of HA protein. The most possible epitopes of NA were located on its N-terminal No. 321-329, 379-386. There were possible B cell epitopes located on the N-terminal No. 46-48, 52-54, 74-78, 136-141, 145-150, 160-164, 194-197, 246-248, 333-343, 426-431. Conclusion The prediction of B cell epitopes of HA and NA proteins with multiple methods benefits the research and development of B cell epitope vaccine against human infection with avian influenza A H7N9 virus.
PMID:
24321078
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/24321078