tetano
Editor, Senior Moderator
Anal Biochem. 2012 Feb 14. [Epub ahead of print]
Precise H1N1 swine influenza detection using stuffer-free multiplex ligation-dependent probe amplification in conformation-sensitive capillary electrophoresis.
Chung B, Shin GW, Hwang HS, Chung YJ, Jung SW, Jung GY.
Source
School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, Korea.
Abstract
The H1N1 influenza virus has spread worldwide to become pandemic. Here, we developed a new method to discriminate various types of influenza A, including H1N1, using stuffer-free multiplex ligation-dependent probe amplification based on a conformation-sensitive separation method, namely capillary electrophoresis-single-strand conformation polymorphism. Unlike conventional methods, our approach precisely detects five relevant gene markers permitting confirmation of infection.
Copyright © 2012. Published by Elsevier Inc.
PMID:
22342882
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22342882
Precise H1N1 swine influenza detection using stuffer-free multiplex ligation-dependent probe amplification in conformation-sensitive capillary electrophoresis.
Chung B, Shin GW, Hwang HS, Chung YJ, Jung SW, Jung GY.
Source
School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, Korea.
Abstract
The H1N1 influenza virus has spread worldwide to become pandemic. Here, we developed a new method to discriminate various types of influenza A, including H1N1, using stuffer-free multiplex ligation-dependent probe amplification based on a conformation-sensitive separation method, namely capillary electrophoresis-single-strand conformation polymorphism. Unlike conventional methods, our approach precisely detects five relevant gene markers permitting confirmation of infection.
Copyright © 2012. Published by Elsevier Inc.
PMID:
22342882
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22342882