tetano
Editor, Senior Moderator
J Immunol. 2018 Oct 17. pii: ji1800981. doi: 10.4049/jimmunol.1800981. [Epub ahead of print]
[h=1]Potential Use of Genetically Engineered Variable Lymphocyte Receptor B Specific to Avian Influenza Virus H9N2.[/h] Im SP[SUP]1[/SUP], Kim J[SUP]1[/SUP], Lee JS[SUP]1[/SUP], Kim SW[SUP]1[/SUP], Jung JW[SUP]1[/SUP], Lazarte JMS[SUP]1[/SUP], Kim JY[SUP]1[/SUP], Kim YR[SUP]1[/SUP], Lee JH[SUP]2[/SUP], Chong RSM[SUP]3[/SUP], Jung TS[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The variable lymphocyte receptor (VLR) B of jawless vertebrates functions as a secreted Ab of jawed vertebrates and has emerged as an alternative Ab with a single polypeptide chain. After observing an upregulated VLRB response in hagfish immunized with avian influenza virus (AIV) subtype H9N2, we screened AIV H9N2-specific VLRB using a mammalian expression system. To improve the binding avidity of the Ag-specific VLRB to the Ag, we enabled multimerization of the VLRB by conjugating it with C-terminal domain of human C4b-binding protein. To dramatically enhance the expression and secretion of the Ag-specific VLRB, we introduced a glycine-serine linker and the murine Ig κ leader sequence. The practical use of the Ag-specific VLRB was also demonstrated through various immunoassays, detected by anti-VLRB Ab (11G5). Finally, we found that the Ag-specific VLRB decreased the infectivity of AIV H9N2. Together, our findings suggest that the generated Ag-specific VLRB could be used for various immunoapplications.
PMID: 30333123 DOI: 10.4049/jimmunol.1800981
[h=1]Potential Use of Genetically Engineered Variable Lymphocyte Receptor B Specific to Avian Influenza Virus H9N2.[/h] Im SP[SUP]1[/SUP], Kim J[SUP]1[/SUP], Lee JS[SUP]1[/SUP], Kim SW[SUP]1[/SUP], Jung JW[SUP]1[/SUP], Lazarte JMS[SUP]1[/SUP], Kim JY[SUP]1[/SUP], Kim YR[SUP]1[/SUP], Lee JH[SUP]2[/SUP], Chong RSM[SUP]3[/SUP], Jung TS[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The variable lymphocyte receptor (VLR) B of jawless vertebrates functions as a secreted Ab of jawed vertebrates and has emerged as an alternative Ab with a single polypeptide chain. After observing an upregulated VLRB response in hagfish immunized with avian influenza virus (AIV) subtype H9N2, we screened AIV H9N2-specific VLRB using a mammalian expression system. To improve the binding avidity of the Ag-specific VLRB to the Ag, we enabled multimerization of the VLRB by conjugating it with C-terminal domain of human C4b-binding protein. To dramatically enhance the expression and secretion of the Ag-specific VLRB, we introduced a glycine-serine linker and the murine Ig κ leader sequence. The practical use of the Ag-specific VLRB was also demonstrated through various immunoassays, detected by anti-VLRB Ab (11G5). Finally, we found that the Ag-specific VLRB decreased the infectivity of AIV H9N2. Together, our findings suggest that the generated Ag-specific VLRB could be used for various immunoapplications.
PMID: 30333123 DOI: 10.4049/jimmunol.1800981