tetano
Editor, Senior Moderator
Int J Clin Exp Med. 2015 Oct 15;8(10):18963-71. eCollection 2015.
[h=1]Polyphylla saponin I has antiviral activity against influenza A virus.[/h] Pu X[SUP]1[/SUP], Ren J[SUP]1[/SUP], Ma X[SUP]1[/SUP], Liu L[SUP]1[/SUP], Yu S[SUP]1[/SUP], Li X[SUP]1[/SUP], Li H[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]OBJECTIVE:[/h] In the present study, the antiviral effects of polyphylla saponin I isolated from Parispolyphylla on influenza A virus are investigated both in vitro and in vivo.
[h=4]METHODS:[/h] Column chromatography and reversed phase liquid chromatography separation technology were used to extract and purify polyphylla saponin I. The purity of polyphylla saponin I was assayed by high performance liquid chromatography. Methyl thiazolyl tetrazolium assay and analyses of cytopathic effects were performed to examine the antiviral activity of polyphylla saponin I upon MDCK cells infected with influenza A virus. Model mice were made by intranasal inoculation of influenza a virus. Mice infected with influenza A virus were orally administered polyphylla saponin I and oseltamivir twice a day for 5 days to study their antiviral efficacy in vivo.
[h=4]RESULTS:[/h] Polyphylla saponin I had no cytotoxicity on MDCK cells at the concentration of 50 μg/mL. Polyphylla saponin I (6.25, 12.5, 25 and 50 μg/mL) and oseltamivir (40 μg/mL) had remarkable inactivation effects on influenza A virus, prevention effects on influenza A virus adsorption on MDCK cells, and inhibitory effects on the reproduction of influenza A virus in MDCK cells. In addition, polyphylla saponin I (5 and 10 mg/kg), and oseltamivir (3 mg/kg) significantly reduced viral hemagglutination titer, improved the pathologic histology of lung tissues, and decreased the mortality of mice infected with influenza A virus. Polyphylla saponin I (5 and 10 mg/kg) prolonged the survival time of mice from 8.5?0.3 days to 13.2?0.5 days, with the prolonged life rates being 49.4% and 55.3%, respectively.
[h=4]CONCLUSION:[/h] Polyphylla saponin I has antiviral activity on influenza A virus both in vitro and in vivo.
[h=4]KEYWORDS:[/h] Influenza A virus; antiviral activity; oseltamivir; polyphylla saponin I
PMID: 26770521 [PubMed]
[h=1]Polyphylla saponin I has antiviral activity against influenza A virus.[/h] Pu X[SUP]1[/SUP], Ren J[SUP]1[/SUP], Ma X[SUP]1[/SUP], Liu L[SUP]1[/SUP], Yu S[SUP]1[/SUP], Li X[SUP]1[/SUP], Li H[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]OBJECTIVE:[/h] In the present study, the antiviral effects of polyphylla saponin I isolated from Parispolyphylla on influenza A virus are investigated both in vitro and in vivo.
[h=4]METHODS:[/h] Column chromatography and reversed phase liquid chromatography separation technology were used to extract and purify polyphylla saponin I. The purity of polyphylla saponin I was assayed by high performance liquid chromatography. Methyl thiazolyl tetrazolium assay and analyses of cytopathic effects were performed to examine the antiviral activity of polyphylla saponin I upon MDCK cells infected with influenza A virus. Model mice were made by intranasal inoculation of influenza a virus. Mice infected with influenza A virus were orally administered polyphylla saponin I and oseltamivir twice a day for 5 days to study their antiviral efficacy in vivo.
[h=4]RESULTS:[/h] Polyphylla saponin I had no cytotoxicity on MDCK cells at the concentration of 50 μg/mL. Polyphylla saponin I (6.25, 12.5, 25 and 50 μg/mL) and oseltamivir (40 μg/mL) had remarkable inactivation effects on influenza A virus, prevention effects on influenza A virus adsorption on MDCK cells, and inhibitory effects on the reproduction of influenza A virus in MDCK cells. In addition, polyphylla saponin I (5 and 10 mg/kg), and oseltamivir (3 mg/kg) significantly reduced viral hemagglutination titer, improved the pathologic histology of lung tissues, and decreased the mortality of mice infected with influenza A virus. Polyphylla saponin I (5 and 10 mg/kg) prolonged the survival time of mice from 8.5?0.3 days to 13.2?0.5 days, with the prolonged life rates being 49.4% and 55.3%, respectively.
[h=4]CONCLUSION:[/h] Polyphylla saponin I has antiviral activity on influenza A virus both in vitro and in vivo.
[h=4]KEYWORDS:[/h] Influenza A virus; antiviral activity; oseltamivir; polyphylla saponin I
PMID: 26770521 [PubMed]