tetano
Editor, Senior Moderator
JCI Insight. 2017 Feb 9;2(3):e87499. doi: 10.1172/jci.insight.87499.
[h=1]Polyfunctional and IFN-γ monofunctional human CD4+ T cell populations are molecularly distinct.[/h] Burel JG[SUP]1[/SUP], Apte SH[SUP]2[/SUP], Groves PL[SUP]2[/SUP], McCarthy JS[SUP]3[/SUP], Doolan DL[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Pathogen-specific polyfunctional T cell responses have been associated with favorable clinical outcomes, but it is not known whether molecular differences exist between polyfunctional and monofunctional cytokine-producing T cells. Here, we report that polyfunctional CD4[SUP]+[/SUP] T cells induced during Plasmodiumfalciparum (P. falciparum) blood-stage infection in humans have a unique transcriptomic profile compared with IFN-γ monofunctional CD4[SUP]+[/SUP] T cells and, thus, are molecularly distinct. The 14-gene signature revealed in P. falciparum-reactive polyfunctional T cells is associated with cytokine signaling and lymphocyte chemotaxis, and systems biology analysis identified IL-27 as an upstream regulator of the polyfunctional gene signature. Importantly, the polyfunctional gene signature is largely conserved in Influenza-reactive polyfunctional CD4[SUP]+[/SUP] T cells, suggesting that polyfunctional T cells have core characteristics independent of pathogen specificity. This study provides the first evidence to our knowledge that consistent molecular differences exist between polyfunctional and monofunctional CD4[SUP]+[/SUP] T cells.
PMID: 28194431 DOI: 10.1172/jci.insight.87499
[PubMed - in process]
[h=1]Polyfunctional and IFN-γ monofunctional human CD4+ T cell populations are molecularly distinct.[/h] Burel JG[SUP]1[/SUP], Apte SH[SUP]2[/SUP], Groves PL[SUP]2[/SUP], McCarthy JS[SUP]3[/SUP], Doolan DL[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Pathogen-specific polyfunctional T cell responses have been associated with favorable clinical outcomes, but it is not known whether molecular differences exist between polyfunctional and monofunctional cytokine-producing T cells. Here, we report that polyfunctional CD4[SUP]+[/SUP] T cells induced during Plasmodiumfalciparum (P. falciparum) blood-stage infection in humans have a unique transcriptomic profile compared with IFN-γ monofunctional CD4[SUP]+[/SUP] T cells and, thus, are molecularly distinct. The 14-gene signature revealed in P. falciparum-reactive polyfunctional T cells is associated with cytokine signaling and lymphocyte chemotaxis, and systems biology analysis identified IL-27 as an upstream regulator of the polyfunctional gene signature. Importantly, the polyfunctional gene signature is largely conserved in Influenza-reactive polyfunctional CD4[SUP]+[/SUP] T cells, suggesting that polyfunctional T cells have core characteristics independent of pathogen specificity. This study provides the first evidence to our knowledge that consistent molecular differences exist between polyfunctional and monofunctional CD4[SUP]+[/SUP] T cells.
PMID: 28194431 DOI: 10.1172/jci.insight.87499
[PubMed - in process]