tetano
Editor, Senior Moderator
Front Immunol. 2019 Jul 11;10:1604. doi: 10.3389/fimmu.2019.01604. eCollection 2019.
[h=1]Poly-γ-Glutamic Acid Complexed With Alum Induces Cross-Protective Immunity of Pandemic H1N1 Vaccine.[/h] Nguyen QT[SUP]1,[/SUP][SUP]2[/SUP], Kwak C[SUP]1,[/SUP][SUP]2[/SUP], Lee WS[SUP]3,[/SUP][SUP]4[/SUP], Kim J[SUP]1,[/SUP][SUP]2[/SUP], Jeong J[SUP]3,[/SUP][SUP]4[/SUP], Sung MH[SUP]5[/SUP], Yang J[SUP]1[/SUP], Poo H[SUP]1,[/SUP][SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The use of a good vaccine adjuvant may induce a higher immunogenicity profile of vaccine antigens. Here, we developed a new adjuvant by combining poly-γ-glutamic acid (γ-PGA) with alum (PGA/Alum) and investigated its ability to enhance the immunogenicity and the cross-reactive efficacy of pandemic H1N1 (pH1N1) influenza vaccine antigen. PGA/Alum enhanced antigen delivery to draining lymph nodes and antigen-specific immunogenicity in mice using OVA as a model antigen. It also greatly increased OVA-specific antibody production, cytotoxic T lymphocyte (CTL) activity, and antibody-dependent cellular cytotoxicity (ADCC). These abilities of PGA/Alum improved the protective efficacy of pH1N1 vaccine antigen by increasing hemagglutination-inhibition titers, enhancing ADCC and CTL activity, and speeding viral clearance following homologous viral challenge. Importantly, the cross-protective efficacy of pH1N1 vaccine against heterologous viruses [A/Puerto Rico/8/34 (H1N1) and A/Hong Kong/1/1968 (H3N2)] was significantly enhanced by PGA/Alum, and cross-reactive ADCC and CTL activities were observed. Together, our results strongly suggest that PGA/Alum may be a promising vaccine adjuvant for preventing influenza and other infectious diseases.
[h=4]KEYWORDS:[/h] antibody-dependent cellular cytotoxicity; cross-protection; cytotoxic T lymphocyte activity; efficacy; influenza virus; vaccine adjuvant
PMID: 31354739 PMCID: PMC6637289 DOI: 10.3389/fimmu.2019.01604
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[h=1]Poly-γ-Glutamic Acid Complexed With Alum Induces Cross-Protective Immunity of Pandemic H1N1 Vaccine.[/h] Nguyen QT[SUP]1,[/SUP][SUP]2[/SUP], Kwak C[SUP]1,[/SUP][SUP]2[/SUP], Lee WS[SUP]3,[/SUP][SUP]4[/SUP], Kim J[SUP]1,[/SUP][SUP]2[/SUP], Jeong J[SUP]3,[/SUP][SUP]4[/SUP], Sung MH[SUP]5[/SUP], Yang J[SUP]1[/SUP], Poo H[SUP]1,[/SUP][SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The use of a good vaccine adjuvant may induce a higher immunogenicity profile of vaccine antigens. Here, we developed a new adjuvant by combining poly-γ-glutamic acid (γ-PGA) with alum (PGA/Alum) and investigated its ability to enhance the immunogenicity and the cross-reactive efficacy of pandemic H1N1 (pH1N1) influenza vaccine antigen. PGA/Alum enhanced antigen delivery to draining lymph nodes and antigen-specific immunogenicity in mice using OVA as a model antigen. It also greatly increased OVA-specific antibody production, cytotoxic T lymphocyte (CTL) activity, and antibody-dependent cellular cytotoxicity (ADCC). These abilities of PGA/Alum improved the protective efficacy of pH1N1 vaccine antigen by increasing hemagglutination-inhibition titers, enhancing ADCC and CTL activity, and speeding viral clearance following homologous viral challenge. Importantly, the cross-protective efficacy of pH1N1 vaccine against heterologous viruses [A/Puerto Rico/8/34 (H1N1) and A/Hong Kong/1/1968 (H3N2)] was significantly enhanced by PGA/Alum, and cross-reactive ADCC and CTL activities were observed. Together, our results strongly suggest that PGA/Alum may be a promising vaccine adjuvant for preventing influenza and other infectious diseases.
[h=4]KEYWORDS:[/h] antibody-dependent cellular cytotoxicity; cross-protection; cytotoxic T lymphocyte activity; efficacy; influenza virus; vaccine adjuvant
PMID: 31354739 PMCID: PMC6637289 DOI: 10.3389/fimmu.2019.01604
Free full text