Giuseppe
Emeritus
Proc Natl Acad Sci U S A. 2008 Sep 30. [Epub ahead of print]
Single-particle kinetics of influenza virus membrane fusion.
Floyd DL, Ragains JR, Skehel JJ, Harrison SC, van Oijen AM. - Department of Biological Chemistry and Molecular Pharmacology, and.
Membrane fusion is an essential step during entry of enveloped viruses into cells.
Conventional fusion assays are generally limited to observation of ensembles of multiple fusion events, confounding more detailed analysis of the sequence of the molecular steps involved.
We have developed an in vitro, two-color fluorescence assay to monitor kinetics of single virus particles fusing with a target bilayer on an essentially fluid support.
Analysis of lipid- and content-mixing trajectories on a particle-by-particle basis provides evidence for multiple, long-lived kinetic intermediates leading to hemifusion, followed by a single, rate-limiting step to pore formation.
We interpret the series of intermediates preceding hemifusion as a result of the requirement that multiple copies of the trimeric hemagglutinin fusion protein be activated to initiate the fusion process.
PMID: 18829437 [PubMed - as supplied by publisher
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Single-particle kinetics of influenza virus membrane fusion.
Floyd DL, Ragains JR, Skehel JJ, Harrison SC, van Oijen AM. - Department of Biological Chemistry and Molecular Pharmacology, and.
Membrane fusion is an essential step during entry of enveloped viruses into cells.
Conventional fusion assays are generally limited to observation of ensembles of multiple fusion events, confounding more detailed analysis of the sequence of the molecular steps involved.
We have developed an in vitro, two-color fluorescence assay to monitor kinetics of single virus particles fusing with a target bilayer on an essentially fluid support.
Analysis of lipid- and content-mixing trajectories on a particle-by-particle basis provides evidence for multiple, long-lived kinetic intermediates leading to hemifusion, followed by a single, rate-limiting step to pore formation.
We interpret the series of intermediates preceding hemifusion as a result of the requirement that multiple copies of the trimeric hemagglutinin fusion protein be activated to initiate the fusion process.
PMID: 18829437 [PubMed - as supplied by publisher
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