tetano
Editor, Senior Moderator
PLoS Pathog
. 2022 Aug 25;18(8):e1010755.
doi: 10.1371/journal.ppat.1010755. eCollection 2022 Aug.
Universal protection against influenza viruses by multi-subtype neuraminidase and M2 ectodomain virus-like particle
Ki-Hye Kim[SUP] 1 [/SUP], Zhuo Li[SUP] 1 [/SUP], Noopur Bhatnagar[SUP] 1 [/SUP], Jeeva Subbiah[SUP] 1 [/SUP], Bo Ryoung Park[SUP] 1 [/SUP], Chong Hyun Shin[SUP] 1 [/SUP], Peter Pushko[SUP] 2 [/SUP], Bao-Zhong Wang[SUP] 1 [/SUP], Sang-Moo Kang[SUP] 1 [/SUP]
Affiliations
Abstract
Annual influenza vaccination is recommended to update the variable hemagglutinin antigens. Here, we first designed a virus-like particle (VLP) displaying consensus multi-neuraminidase (NA) subtypes (cN1, cN2, B cNA) and M2 ectodomain (M2e) tandem repeat (m-cNA-M2e VLP). Vaccination of mice with m-cNA-M2e VLP induced broad NA inhibition (NAI), and M2e antibodies as well as interferon-gamma secreting T cell responses. Mice vaccinated with m-cNA-M2e VLP were protected against influenza A (H1N1, H5N1, H3N2, H9N2, H7N9) and influenza B (Yamagata and Victoria lineage) viruses containing substantial antigenic variations. Protective immune contributors include cellular and humoral immunity as well as antibody-dependent cellular cytotoxicity. Furthermore, comparable cross protection by m-cNA-M2e VLP vaccination was induced in aged mice. This study supports a novel strategy of developing a universal vaccine against influenza A and B viruses potentially in both young and aged populations by inducing multi-NA subtype and M2e immunity with a single VLP entity.
. 2022 Aug 25;18(8):e1010755.
doi: 10.1371/journal.ppat.1010755. eCollection 2022 Aug.
Universal protection against influenza viruses by multi-subtype neuraminidase and M2 ectodomain virus-like particle
Ki-Hye Kim[SUP] 1 [/SUP], Zhuo Li[SUP] 1 [/SUP], Noopur Bhatnagar[SUP] 1 [/SUP], Jeeva Subbiah[SUP] 1 [/SUP], Bo Ryoung Park[SUP] 1 [/SUP], Chong Hyun Shin[SUP] 1 [/SUP], Peter Pushko[SUP] 2 [/SUP], Bao-Zhong Wang[SUP] 1 [/SUP], Sang-Moo Kang[SUP] 1 [/SUP]
Affiliations
- PMID: 36006890
- DOI: 10.1371/journal.ppat.1010755
Abstract
Annual influenza vaccination is recommended to update the variable hemagglutinin antigens. Here, we first designed a virus-like particle (VLP) displaying consensus multi-neuraminidase (NA) subtypes (cN1, cN2, B cNA) and M2 ectodomain (M2e) tandem repeat (m-cNA-M2e VLP). Vaccination of mice with m-cNA-M2e VLP induced broad NA inhibition (NAI), and M2e antibodies as well as interferon-gamma secreting T cell responses. Mice vaccinated with m-cNA-M2e VLP were protected against influenza A (H1N1, H5N1, H3N2, H9N2, H7N9) and influenza B (Yamagata and Victoria lineage) viruses containing substantial antigenic variations. Protective immune contributors include cellular and humoral immunity as well as antibody-dependent cellular cytotoxicity. Furthermore, comparable cross protection by m-cNA-M2e VLP vaccination was induced in aged mice. This study supports a novel strategy of developing a universal vaccine against influenza A and B viruses potentially in both young and aged populations by inducing multi-NA subtype and M2e immunity with a single VLP entity.