tetano
Editor, Senior Moderator
PLoS Pathog
. 2021 Nov 19;17(11):e1009743.
doi: 10.1371/journal.ppat.1009743. Online ahead of print.
Phosphatidylserine receptors enhance SARS-CoV-2 infection
Dana Bohan[SUP] 1 [/SUP], Hanora Van Ert[SUP] 1 [/SUP], Natalie Ruggio[SUP] 1 [/SUP], Kai J Rogers[SUP] 1 [/SUP], Mohammad Badreddine[SUP] 1 [/SUP], José A Aguilar Briseño[SUP] 1 [/SUP], Jonah M Elliff[SUP] 1 [/SUP], Roberth Anthony Rojas Chavez[SUP] 1 [/SUP], Boning Gao[SUP] 2 [/SUP], Tomasz Stokowy[SUP] 3 [/SUP], Eleni Christakou[SUP] 3 4 [/SUP], Petri Kursula[SUP] 3 5 [/SUP], David Micklem[SUP] 4 [/SUP], Gro Gausdal[SUP] 4 [/SUP], Hillel Haim[SUP] 1 [/SUP], John Minna[SUP] 2 [/SUP], James B Lorens[SUP] 3 [/SUP], Wendy Maury[SUP] 1 [/SUP]
Affiliations
Abstract
Phosphatidylserine (PS) receptors enhance infection of many enveloped viruses through virion-associated PS binding that is termed apoptotic mimicry. Here we show that this broadly shared uptake mechanism is utilized by SARS-CoV-2 in cells that express low surface levels of ACE2. Expression of members of the TIM (TIM-1 and TIM-4) and TAM (AXL) families of PS receptors enhance SARS-CoV-2 binding to cells, facilitate internalization of fluorescently-labeled virions and increase ACE2-dependent infection of SARS-CoV-2; however, PS receptors alone did not mediate infection. We were unable to detect direct interactions of the PS receptor AXL with purified SARS-CoV-2 spike, contrary to a previous report. Instead, our studies indicate that the PS receptors interact with PS on the surface of SARS-CoV-2 virions. In support of this, we demonstrate that: 1) significant quantities of PS are located on the outer leaflet of SARS-CoV-2 virions, 2) PS liposomes, but not phosphatidylcholine liposomes, reduced entry of VSV/Spike pseudovirions and 3) an established mutant of TIM-1 which does not bind to PS is unable to facilitate entry of SARS-CoV-2. As AXL is an abundant PS receptor on a number of airway lines, we evaluated small molecule inhibitors of AXL signaling such as bemcentinib for their ability to inhibit SARS-CoV-2 infection. Bemcentinib robustly inhibited virus infection of Vero E6 cells as well as multiple human lung cell lines that expressed AXL. This inhibition correlated well with inhibitors that block endosomal acidification and cathepsin activity, consistent with AXL-mediated uptake of SARS-CoV-2 into the endosomal compartment. We extended our observations to the related betacoronavirus mouse hepatitis virus (MHV), showing that inhibition or ablation of AXL reduces MHV infection of murine cells. In total, our findings provide evidence that PS receptors facilitate infection of the pandemic coronavirus SARS-CoV-2 and suggest that inhibition of the PS receptor AXL has therapeutic potential against SARS-CoV-2.
. 2021 Nov 19;17(11):e1009743.
doi: 10.1371/journal.ppat.1009743. Online ahead of print.
Phosphatidylserine receptors enhance SARS-CoV-2 infection
Dana Bohan[SUP] 1 [/SUP], Hanora Van Ert[SUP] 1 [/SUP], Natalie Ruggio[SUP] 1 [/SUP], Kai J Rogers[SUP] 1 [/SUP], Mohammad Badreddine[SUP] 1 [/SUP], José A Aguilar Briseño[SUP] 1 [/SUP], Jonah M Elliff[SUP] 1 [/SUP], Roberth Anthony Rojas Chavez[SUP] 1 [/SUP], Boning Gao[SUP] 2 [/SUP], Tomasz Stokowy[SUP] 3 [/SUP], Eleni Christakou[SUP] 3 4 [/SUP], Petri Kursula[SUP] 3 5 [/SUP], David Micklem[SUP] 4 [/SUP], Gro Gausdal[SUP] 4 [/SUP], Hillel Haim[SUP] 1 [/SUP], John Minna[SUP] 2 [/SUP], James B Lorens[SUP] 3 [/SUP], Wendy Maury[SUP] 1 [/SUP]
Affiliations
- PMID: 34797899
- DOI: 10.1371/journal.ppat.1009743
Abstract
Phosphatidylserine (PS) receptors enhance infection of many enveloped viruses through virion-associated PS binding that is termed apoptotic mimicry. Here we show that this broadly shared uptake mechanism is utilized by SARS-CoV-2 in cells that express low surface levels of ACE2. Expression of members of the TIM (TIM-1 and TIM-4) and TAM (AXL) families of PS receptors enhance SARS-CoV-2 binding to cells, facilitate internalization of fluorescently-labeled virions and increase ACE2-dependent infection of SARS-CoV-2; however, PS receptors alone did not mediate infection. We were unable to detect direct interactions of the PS receptor AXL with purified SARS-CoV-2 spike, contrary to a previous report. Instead, our studies indicate that the PS receptors interact with PS on the surface of SARS-CoV-2 virions. In support of this, we demonstrate that: 1) significant quantities of PS are located on the outer leaflet of SARS-CoV-2 virions, 2) PS liposomes, but not phosphatidylcholine liposomes, reduced entry of VSV/Spike pseudovirions and 3) an established mutant of TIM-1 which does not bind to PS is unable to facilitate entry of SARS-CoV-2. As AXL is an abundant PS receptor on a number of airway lines, we evaluated small molecule inhibitors of AXL signaling such as bemcentinib for their ability to inhibit SARS-CoV-2 infection. Bemcentinib robustly inhibited virus infection of Vero E6 cells as well as multiple human lung cell lines that expressed AXL. This inhibition correlated well with inhibitors that block endosomal acidification and cathepsin activity, consistent with AXL-mediated uptake of SARS-CoV-2 into the endosomal compartment. We extended our observations to the related betacoronavirus mouse hepatitis virus (MHV), showing that inhibition or ablation of AXL reduces MHV infection of murine cells. In total, our findings provide evidence that PS receptors facilitate infection of the pandemic coronavirus SARS-CoV-2 and suggest that inhibition of the PS receptor AXL has therapeutic potential against SARS-CoV-2.