tetano
Editor, Senior Moderator
PLoS Pathog
. 2025 Nov 19;21(11):e1013703.
doi: 10.1371/journal.ppat.1013703. eCollection 2025 Nov. Non-canonical Metatranscriptomic analysis of COVID-19 and Dengue reveals an expanded microbial and AMR landscape in COVID-19 mortality patients
Aanchal Yadav[SUP] 1 2 [/SUP], Raiyan Ali[SUP] 1 [/SUP], Priti Devi[SUP] 1 2 [/SUP], Pallawi Kumari[SUP] 1 3 [/SUP], Jyoti Soni[SUP] 1 2 [/SUP], Garima[SUP] 1 2 [/SUP], Bansidhar Tarai[SUP] 4 [/SUP], Sandeep Budhiraja[SUP] 4 [/SUP], Uzma Shamim[SUP] 1 5 [/SUP], Rajesh Pandey[SUP] 1 2 [/SUP]
Affiliations
AMR is a growing concern in viral infections, where microbiome shifts contribute to resistance gene dissemination. While dengue and COVID-19, caused by ssRNA viruses, are not bacterial-driven, their resistome and microbial communities influence disease progression and AMR burden. This study analyzes the resistome and microbiome in 251 COVID-19 and 112 dengue patients using non-canonical metatranscriptomics. By mapping antimicrobial resistance genes (ARGs) and their transcriptionally active microbes (TAMs) hosts, we uncover greater ARG burden in COVID-19, particularly during mortality, with a diverse set of associated TAMs compared to dengue. MDR genes were prevalent, with beta-lactamase ARGs commonly detected in both infections. COVID-19 exhibited higher carbapenemase resistance genes (NDM, OXA, VIM), while dengue was associated with TEM variants. Escherichia coli and Klebsiella pneumoniae were dominant ARG hosts, with Acinetobacter baumannii in COVID-19 mortality and Bacillus cereus in severe dengue. These findings highlight resistome dynamics and emphasize the need for AMR surveillance in high-burden infections.
. 2025 Nov 19;21(11):e1013703.
doi: 10.1371/journal.ppat.1013703. eCollection 2025 Nov. Non-canonical Metatranscriptomic analysis of COVID-19 and Dengue reveals an expanded microbial and AMR landscape in COVID-19 mortality patients
Aanchal Yadav[SUP] 1 2 [/SUP], Raiyan Ali[SUP] 1 [/SUP], Priti Devi[SUP] 1 2 [/SUP], Pallawi Kumari[SUP] 1 3 [/SUP], Jyoti Soni[SUP] 1 2 [/SUP], Garima[SUP] 1 2 [/SUP], Bansidhar Tarai[SUP] 4 [/SUP], Sandeep Budhiraja[SUP] 4 [/SUP], Uzma Shamim[SUP] 1 5 [/SUP], Rajesh Pandey[SUP] 1 2 [/SUP]
Affiliations
- PMID: 41259398
- DOI: 10.1371/journal.ppat.1013703
AMR is a growing concern in viral infections, where microbiome shifts contribute to resistance gene dissemination. While dengue and COVID-19, caused by ssRNA viruses, are not bacterial-driven, their resistome and microbial communities influence disease progression and AMR burden. This study analyzes the resistome and microbiome in 251 COVID-19 and 112 dengue patients using non-canonical metatranscriptomics. By mapping antimicrobial resistance genes (ARGs) and their transcriptionally active microbes (TAMs) hosts, we uncover greater ARG burden in COVID-19, particularly during mortality, with a diverse set of associated TAMs compared to dengue. MDR genes were prevalent, with beta-lactamase ARGs commonly detected in both infections. COVID-19 exhibited higher carbapenemase resistance genes (NDM, OXA, VIM), while dengue was associated with TEM variants. Escherichia coli and Klebsiella pneumoniae were dominant ARG hosts, with Acinetobacter baumannii in COVID-19 mortality and Bacillus cereus in severe dengue. These findings highlight resistome dynamics and emphasize the need for AMR surveillance in high-burden infections.