tetano
Editor, Senior Moderator
PLoS Pathog
. 2020 Aug 27;16(8):e1008705.
doi: 10.1371/journal.ppat.1008705. eCollection 2020 Aug.
Development and evaluation of a rapid CRISPR-based diagnostic for COVID-19
Tieying Hou[SUP] 1 [/SUP], Weiqi Zeng[SUP] 2 3 [/SUP], Minling Yang[SUP] 2 [/SUP], Wenjing Chen[SUP] 2 [/SUP], Lili Ren[SUP] 4 [/SUP], Jingwen Ai[SUP] 5 [/SUP], Ji Wu[SUP] 1 [/SUP], Yalong Liao[SUP] 1 [/SUP], Xuejing Gou[SUP] 2 [/SUP], Yongjun Li[SUP] 2 [/SUP], Xiaorui Wang[SUP] 2 [/SUP], Hang Su[SUP] 2 [/SUP], Bing Gu[SUP] 6 7 [/SUP], Jianwei Wang[SUP] 4 [/SUP], Teng Xu[SUP] 2 3 [/SUP]
Affiliations
Abstract
The recent outbreak of human infections caused by SARS-CoV-2, the third zoonotic coronavirus has raised great public health concern globally. Rapid and accurate diagnosis of this novel pathogen posts great challenges not only clinically but also technologically. Metagenomic next-generation sequencing (mNGS) and reverse-transcription PCR (RT-PCR) have been the most commonly used molecular methodologies. However, each has their own limitations. In this study, we developed an isothermal, CRISPR-based diagnostic for COVID-19 with near single-copy sensitivity. The diagnostic performances of all three technology platforms were also compared. Our study aimed to provide more insights into the molecular detection of SARS-CoV-2, and also to present a novel diagnostic option for this new emerging virus.
. 2020 Aug 27;16(8):e1008705.
doi: 10.1371/journal.ppat.1008705. eCollection 2020 Aug.
Development and evaluation of a rapid CRISPR-based diagnostic for COVID-19
Tieying Hou[SUP] 1 [/SUP], Weiqi Zeng[SUP] 2 3 [/SUP], Minling Yang[SUP] 2 [/SUP], Wenjing Chen[SUP] 2 [/SUP], Lili Ren[SUP] 4 [/SUP], Jingwen Ai[SUP] 5 [/SUP], Ji Wu[SUP] 1 [/SUP], Yalong Liao[SUP] 1 [/SUP], Xuejing Gou[SUP] 2 [/SUP], Yongjun Li[SUP] 2 [/SUP], Xiaorui Wang[SUP] 2 [/SUP], Hang Su[SUP] 2 [/SUP], Bing Gu[SUP] 6 7 [/SUP], Jianwei Wang[SUP] 4 [/SUP], Teng Xu[SUP] 2 3 [/SUP]
Affiliations
- PMID: 32853291
- DOI: 10.1371/journal.ppat.1008705
Abstract
The recent outbreak of human infections caused by SARS-CoV-2, the third zoonotic coronavirus has raised great public health concern globally. Rapid and accurate diagnosis of this novel pathogen posts great challenges not only clinically but also technologically. Metagenomic next-generation sequencing (mNGS) and reverse-transcription PCR (RT-PCR) have been the most commonly used molecular methodologies. However, each has their own limitations. In this study, we developed an isothermal, CRISPR-based diagnostic for COVID-19 with near single-copy sensitivity. The diagnostic performances of all three technology platforms were also compared. Our study aimed to provide more insights into the molecular detection of SARS-CoV-2, and also to present a novel diagnostic option for this new emerging virus.