tetano
Editor, Senior Moderator
PLoS One
. 2023 Jun 9;18(6):e0287107.
doi: 10.1371/journal.pone.0287107. eCollection 2023. Standardization of in-house anti-IgG and IgA ELISAs for the detection of COVID-19
Kamonthip Rungrojcharoenkit[SUP] 1 [/SUP], Rungarun Suthangkornkul[SUP] 1 [/SUP], Darunee Utennam[SUP] 2 [/SUP], Darunee Buddhari[SUP] 1 [/SUP], Soontorn Pinpaiboon[SUP] 3 [/SUP], Duangrat Mongkolsirichaikul[SUP] 1 [/SUP], Stefan Fernandez[SUP] 1 [/SUP], Anthony R Jones[SUP] 1 [/SUP], Thomas S Cotrone[SUP] 1 [/SUP], Taweewun Hunsawong[SUP] 1 [/SUP]
Affiliations
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19). RT-PCR detection of viral RNA represents the gold standard method for diagnosis of COVID-19. However, multiple diagnostic tests are needed for acute disease diagnosis and assessing immunity during the COVID-19 outbreak. Here, we developed in-house anti-RBD IgG and IgA enzyme-linked immunosorbent assays (ELISAs) using a well-defined serum sample panel for screening and identification of human SARS-CoV-2 infection. We found that our in-house anti-SARS-CoV-2 IgG ELISA displayed a 93.5% sensitivity and 98.8% specificity whereas our in-house anti-SARS-CoV-2 IgA ELISA provided assay sensitivity and specificity at 89.5% and 99.4%, respectively. The agreement kappa values of our in-house anti-SARS-CoV-2 IgG and IgA ELISA assays were deemed to be excellent and fair, respectively, when compared to RT-PCR and excellent for both assays when compared to Euroimmun anti-SARS-CoV-2 IgG and IgA ELISAs. These data indicate that our in-house anti-SARS-CoV-2 IgG and IgA ELISAs are compatible performing assays for the detection of SARS-CoV-2 infection.
. 2023 Jun 9;18(6):e0287107.
doi: 10.1371/journal.pone.0287107. eCollection 2023. Standardization of in-house anti-IgG and IgA ELISAs for the detection of COVID-19
Kamonthip Rungrojcharoenkit[SUP] 1 [/SUP], Rungarun Suthangkornkul[SUP] 1 [/SUP], Darunee Utennam[SUP] 2 [/SUP], Darunee Buddhari[SUP] 1 [/SUP], Soontorn Pinpaiboon[SUP] 3 [/SUP], Duangrat Mongkolsirichaikul[SUP] 1 [/SUP], Stefan Fernandez[SUP] 1 [/SUP], Anthony R Jones[SUP] 1 [/SUP], Thomas S Cotrone[SUP] 1 [/SUP], Taweewun Hunsawong[SUP] 1 [/SUP]
Affiliations
- PMID: 37294808
- DOI: 10.1371/journal.pone.0287107
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19). RT-PCR detection of viral RNA represents the gold standard method for diagnosis of COVID-19. However, multiple diagnostic tests are needed for acute disease diagnosis and assessing immunity during the COVID-19 outbreak. Here, we developed in-house anti-RBD IgG and IgA enzyme-linked immunosorbent assays (ELISAs) using a well-defined serum sample panel for screening and identification of human SARS-CoV-2 infection. We found that our in-house anti-SARS-CoV-2 IgG ELISA displayed a 93.5% sensitivity and 98.8% specificity whereas our in-house anti-SARS-CoV-2 IgA ELISA provided assay sensitivity and specificity at 89.5% and 99.4%, respectively. The agreement kappa values of our in-house anti-SARS-CoV-2 IgG and IgA ELISA assays were deemed to be excellent and fair, respectively, when compared to RT-PCR and excellent for both assays when compared to Euroimmun anti-SARS-CoV-2 IgG and IgA ELISAs. These data indicate that our in-house anti-SARS-CoV-2 IgG and IgA ELISAs are compatible performing assays for the detection of SARS-CoV-2 infection.