tetano
Editor, Senior Moderator
PLoS One
. 2021 Jul 26;16(7):e0253551.
doi: 10.1371/journal.pone.0253551. eCollection 2021.
Predicting the efficacy of COVID-19 convalescent plasma donor units with the Lumit Dx anti-receptor binding domain assay
Sanath Kumar Janaka[SUP] 1 [/SUP], Natasha M Clark[SUP] 1 [/SUP], David T Evans[SUP] 1 2 [/SUP], Huihui Mou[SUP] 3 [/SUP], Michael Farzan[SUP] 3 [/SUP], Joseph P Connor[SUP] 1 [/SUP]
Affiliations
Abstract
Background: The novel coronavirus SARS-CoV2 that causes COVID-19 has resulted in the death of more than 2.5 million people, but no cure exists. Although passive immunization with COVID-19 convalescent plasma (CCP) provides a safe and viable therapeutic option, the selection of optimal units for therapy in a timely fashion remains a barrier.
Study design and methods: Since virus neutralization is a necessary characteristic of plasma that can benefit recipients, the neutralizing titers of plasma samples were measured using a retroviral-pseudotype assay. Binding antibody titers to the spike (S) protein were also determined by a clinically available serological assay (Ortho-Vitros total IG), and an in-house ELISA. The results of these assays were compared to a measurement of antibodies directed to the receptor binding domain (RBD) of the SARS-CoV2 S protein (Promega Lumit Dx).
Results: All measures of antibodies were highly variable, but correlated, to different degrees, with each other. However, the anti-RBD antibodies correlated with viral neutralizing titers to a greater extent than the other antibody assays.
Discussion: Our observations support the use of an anti-RBD assay such as the Lumit Dx assay, as an optimal predictor of the neutralization capability of CCP.
. 2021 Jul 26;16(7):e0253551.
doi: 10.1371/journal.pone.0253551. eCollection 2021.
Predicting the efficacy of COVID-19 convalescent plasma donor units with the Lumit Dx anti-receptor binding domain assay
Sanath Kumar Janaka[SUP] 1 [/SUP], Natasha M Clark[SUP] 1 [/SUP], David T Evans[SUP] 1 2 [/SUP], Huihui Mou[SUP] 3 [/SUP], Michael Farzan[SUP] 3 [/SUP], Joseph P Connor[SUP] 1 [/SUP]
Affiliations
- PMID: 34310603
- DOI: 10.1371/journal.pone.0253551
Abstract
Background: The novel coronavirus SARS-CoV2 that causes COVID-19 has resulted in the death of more than 2.5 million people, but no cure exists. Although passive immunization with COVID-19 convalescent plasma (CCP) provides a safe and viable therapeutic option, the selection of optimal units for therapy in a timely fashion remains a barrier.
Study design and methods: Since virus neutralization is a necessary characteristic of plasma that can benefit recipients, the neutralizing titers of plasma samples were measured using a retroviral-pseudotype assay. Binding antibody titers to the spike (S) protein were also determined by a clinically available serological assay (Ortho-Vitros total IG), and an in-house ELISA. The results of these assays were compared to a measurement of antibodies directed to the receptor binding domain (RBD) of the SARS-CoV2 S protein (Promega Lumit Dx).
Results: All measures of antibodies were highly variable, but correlated, to different degrees, with each other. However, the anti-RBD antibodies correlated with viral neutralizing titers to a greater extent than the other antibody assays.
Discussion: Our observations support the use of an anti-RBD assay such as the Lumit Dx assay, as an optimal predictor of the neutralization capability of CCP.