tetano
Editor, Senior Moderator
PLoS One
. 2020 Jul 8;15(7):e0235706.
doi: 10.1371/journal.pone.0235706. eCollection 2020.
Placenta-specific 8 Limits IFNγ Production by CD4 T Cells in Vitro and Promotes Establishment of Influenza-Specific CD8 T Cells in Vivo
Chris D Slade[SUP] 1 [/SUP], Katie L Reagin[SUP] 2 [/SUP], Hari G Lakshmanan[SUP] 2 [/SUP], Kimberly D Klonowski[SUP] 1 2 [/SUP], Wendy T Watford[SUP] 1 [/SUP]
Affiliations
Abstract
During type 1 immune responses, CD4 T helper 1 (Th1) cells and CD8 T cells are activated via IL-12 and contribute to the elimination of intracellular pathogens through interferon gamma (IFNγ) production. In this study, we identified Placenta-specific 8 (Plac8) as a gene that is uniquely expressed in Th1 CD4 T cells relative to other CD4 T cell subsets and hypothesized that Plac8 may represent a novel therapeutic target in Th1 CD4 T cells. First, we determined that Plac8 mRNA in CD4 T cells was induced following IL-12 stimulation via an indirect route that required new protein synthesis. Upon evaluating the functional relevance of Plac8 expression in Th1 CD4 T cells, we discovered that Plac8 was important for suppressing IFNγ mRNA and protein production by CD4 T cells 24 hours after IL-12 stimulation, however Plac8 did not contribute to pathogenic CD4 T cell function during two models of intestinal inflammation. We also noted relatively high basal expression of Plac8 in CD8 T cells which could be further induced following IL-12 stimulation in CD8 T cells. Furthermore, Plac8 expression was important for establishing an optimal CD8 T cell response against influenza A virus via a T cell-intrinsic manner. Altogether, these results implicate Plac8 as a potential regulator of Th1 CD4 and CD8 T cell responses during Th1 T cell-driven inflammation.
. 2020 Jul 8;15(7):e0235706.
doi: 10.1371/journal.pone.0235706. eCollection 2020.
Placenta-specific 8 Limits IFNγ Production by CD4 T Cells in Vitro and Promotes Establishment of Influenza-Specific CD8 T Cells in Vivo
Chris D Slade[SUP] 1 [/SUP], Katie L Reagin[SUP] 2 [/SUP], Hari G Lakshmanan[SUP] 2 [/SUP], Kimberly D Klonowski[SUP] 1 2 [/SUP], Wendy T Watford[SUP] 1 [/SUP]
Affiliations
- PMID: 32639988
- DOI: 10.1371/journal.pone.0235706
Abstract
During type 1 immune responses, CD4 T helper 1 (Th1) cells and CD8 T cells are activated via IL-12 and contribute to the elimination of intracellular pathogens through interferon gamma (IFNγ) production. In this study, we identified Placenta-specific 8 (Plac8) as a gene that is uniquely expressed in Th1 CD4 T cells relative to other CD4 T cell subsets and hypothesized that Plac8 may represent a novel therapeutic target in Th1 CD4 T cells. First, we determined that Plac8 mRNA in CD4 T cells was induced following IL-12 stimulation via an indirect route that required new protein synthesis. Upon evaluating the functional relevance of Plac8 expression in Th1 CD4 T cells, we discovered that Plac8 was important for suppressing IFNγ mRNA and protein production by CD4 T cells 24 hours after IL-12 stimulation, however Plac8 did not contribute to pathogenic CD4 T cell function during two models of intestinal inflammation. We also noted relatively high basal expression of Plac8 in CD8 T cells which could be further induced following IL-12 stimulation in CD8 T cells. Furthermore, Plac8 expression was important for establishing an optimal CD8 T cell response against influenza A virus via a T cell-intrinsic manner. Altogether, these results implicate Plac8 as a potential regulator of Th1 CD4 and CD8 T cell responses during Th1 T cell-driven inflammation.