tetano
Editor, Senior Moderator
PLoS One
. 2021 Jun 23;16(6):e0252317.
doi: 10.1371/journal.pone.0252317. eCollection 2021.
Performance of the EUROIMMUN Anti-SARS-CoV-2 ELISA Assay for detection of IgA and IgG antibodies in South Africa
Maemu P Gededzha[SUP] 1 2 [/SUP], Nakampe Mampeule[SUP] 1 2 [/SUP], Sarika Jugwanth[SUP] 1 2 [/SUP], Nontobeko Zwane[SUP] 1 [/SUP], Anura David[SUP] 3 [/SUP], Wendy A Burgers[SUP] 4 5 6 [/SUP], Jonathan M Blackburn[SUP] 6 7 [/SUP], Jurette S Grove[SUP] 2 8 [/SUP], Jaya A George[SUP] 2 8 [/SUP], Ian Sanne[SUP] 9 [/SUP], Lesley Scott[SUP] 2 3 [/SUP], Wendy Stevens[SUP] 2 3 [/SUP], Elizabeth S Mayne[SUP] 1 2 [/SUP]
Affiliations
Abstract
Severe Acute Respiratory Syndrome-Coronavirus 2 (SARS-CoV-2) has been identified as the causative agent for causing the clinical syndrome of COVID -19. Accurate detection of SARS-CoV-2 infection is not only important for management of infected individuals but also to break the chain of transmission. South Africa is the current epicenter of SARS-CoV-2 infection in Africa. To optimize the diagnostic algorithm for SARS-CoV-2 in the South African setting, the study aims to evaluate the diagnostic performance of the EUROIMMUN Anti-SARS-CoV-2 assays. This study reported the performance of EUROIMMUN enzyme-linked immunosorbent assay (ELISA) for semi-quantitative detection of IgA and IgG antibodies in serum and plasma samples targeting the recombinant S1 domain of the SARS-CoV-2 spike protein as antigen. Samples were collected from 391 individuals who had tested positive for SARS-CoV-2 and 139 SARS CoV-2 negative controls. Samples were stratified by number of days' post-PCR diagnosis and symptoms. The sensitivity of EUROIMMUN IgG was 64.1% (95% CI: 59.1-69.0%) and 74.3% (95% CI: 69.6-78.6%) for IgA and the specificity was lower for IgA [84.2% (95% CI: 77-89.2%)] than IgG [95.2% (95% CI: 90.8-98.4%)]. The EUROIMMUN Anti-SARS-CoV-2 ELISA Assay sensitivity was higher for IgA but low for IgG and improved for both assays in symptomatic individuals and at later timepoints post PCR diagnosis.
. 2021 Jun 23;16(6):e0252317.
doi: 10.1371/journal.pone.0252317. eCollection 2021.
Performance of the EUROIMMUN Anti-SARS-CoV-2 ELISA Assay for detection of IgA and IgG antibodies in South Africa
Maemu P Gededzha[SUP] 1 2 [/SUP], Nakampe Mampeule[SUP] 1 2 [/SUP], Sarika Jugwanth[SUP] 1 2 [/SUP], Nontobeko Zwane[SUP] 1 [/SUP], Anura David[SUP] 3 [/SUP], Wendy A Burgers[SUP] 4 5 6 [/SUP], Jonathan M Blackburn[SUP] 6 7 [/SUP], Jurette S Grove[SUP] 2 8 [/SUP], Jaya A George[SUP] 2 8 [/SUP], Ian Sanne[SUP] 9 [/SUP], Lesley Scott[SUP] 2 3 [/SUP], Wendy Stevens[SUP] 2 3 [/SUP], Elizabeth S Mayne[SUP] 1 2 [/SUP]
Affiliations
- PMID: 34161348
- DOI: 10.1371/journal.pone.0252317
Abstract
Severe Acute Respiratory Syndrome-Coronavirus 2 (SARS-CoV-2) has been identified as the causative agent for causing the clinical syndrome of COVID -19. Accurate detection of SARS-CoV-2 infection is not only important for management of infected individuals but also to break the chain of transmission. South Africa is the current epicenter of SARS-CoV-2 infection in Africa. To optimize the diagnostic algorithm for SARS-CoV-2 in the South African setting, the study aims to evaluate the diagnostic performance of the EUROIMMUN Anti-SARS-CoV-2 assays. This study reported the performance of EUROIMMUN enzyme-linked immunosorbent assay (ELISA) for semi-quantitative detection of IgA and IgG antibodies in serum and plasma samples targeting the recombinant S1 domain of the SARS-CoV-2 spike protein as antigen. Samples were collected from 391 individuals who had tested positive for SARS-CoV-2 and 139 SARS CoV-2 negative controls. Samples were stratified by number of days' post-PCR diagnosis and symptoms. The sensitivity of EUROIMMUN IgG was 64.1% (95% CI: 59.1-69.0%) and 74.3% (95% CI: 69.6-78.6%) for IgA and the specificity was lower for IgA [84.2% (95% CI: 77-89.2%)] than IgG [95.2% (95% CI: 90.8-98.4%)]. The EUROIMMUN Anti-SARS-CoV-2 ELISA Assay sensitivity was higher for IgA but low for IgG and improved for both assays in symptomatic individuals and at later timepoints post PCR diagnosis.