tetano
Editor, Senior Moderator
PLoS One
. 2023 Apr 13;18(4):e0283589.
doi: 10.1371/journal.pone.0283589. eCollection 2023.
Mapping CircRNA-miRNA-mRNA regulatory axis identifies hsa_circ_0080942 and hsa_circ_0080135 as a potential theranostic agents for SARS-CoV-2 infection
Hassan Ayaz[SUP] 1 [/SUP], Nouman Aslam[SUP] 1 [/SUP], Faryal Mehwish Awan[SUP] 1 [/SUP], Rabea Basri[SUP] 1 [/SUP], Bisma Rauff[SUP] 2 [/SUP], Badr Alzahrani[SUP] 3 [/SUP], Muhammad Arif[SUP] 1 [/SUP], Aqsa Ikram[SUP] 4 [/SUP], Ayesha Obaid[SUP] 1 [/SUP], Anam Naz[SUP] 4 [/SUP], Sadiq Noor Khan[SUP] 1 [/SUP], Burton B Yang[SUP] 5 6 7 [/SUP], Azhar Nazir[SUP] 1 [/SUP]
Affiliations
Abstract
Non-coding RNAs (ncRNAs) can control the flux of genetic information; affect RNA stability and play crucial roles in mediating epigenetic modifications. A number of studies have highlighted the potential roles of both virus-encoded and host-encoded ncRNAs in viral infections, transmission and therapeutics. However, the role of an emerging type of non-coding transcript, circular RNA (circRNA) in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has not been fully elucidated so far. Moreover, the potential pathogenic role of circRNA-miRNA-mRNA regulatory axis has not been fully explored as yet. The current study aimed to holistically map the regulatory networks driven by SARS-CoV-2 related circRNAs, miRNAs and mRNAs to uncover plausible interactions and interplay amongst them in order to explore possible therapeutic options in SARS-CoV-2 infection. Patient datasets were analyzed systematically in a unified approach to explore circRNA, miRNA, and mRNA expression profiles. CircRNA-miRNA-mRNA network was constructed based on cytokine storm related circRNAs forming a total of 165 circRNA-miRNA-mRNA pairs. This study implies the potential regulatory role of the obtained circRNA-miRNA-mRNA network and proposes that two differentially expressed circRNAs hsa_circ_0080942 and hsa_circ_0080135 might serve as a potential theranostic agents for SARS-CoV-2 infection. Collectively, the results shed light on the functional role of circRNAs as ceRNAs to sponge miRNA and regulate mRNA expression during SARS-CoV-2 infection.
. 2023 Apr 13;18(4):e0283589.
doi: 10.1371/journal.pone.0283589. eCollection 2023.
Mapping CircRNA-miRNA-mRNA regulatory axis identifies hsa_circ_0080942 and hsa_circ_0080135 as a potential theranostic agents for SARS-CoV-2 infection
Hassan Ayaz[SUP] 1 [/SUP], Nouman Aslam[SUP] 1 [/SUP], Faryal Mehwish Awan[SUP] 1 [/SUP], Rabea Basri[SUP] 1 [/SUP], Bisma Rauff[SUP] 2 [/SUP], Badr Alzahrani[SUP] 3 [/SUP], Muhammad Arif[SUP] 1 [/SUP], Aqsa Ikram[SUP] 4 [/SUP], Ayesha Obaid[SUP] 1 [/SUP], Anam Naz[SUP] 4 [/SUP], Sadiq Noor Khan[SUP] 1 [/SUP], Burton B Yang[SUP] 5 6 7 [/SUP], Azhar Nazir[SUP] 1 [/SUP]
Affiliations
- PMID: 37053191
- DOI: 10.1371/journal.pone.0283589
Abstract
Non-coding RNAs (ncRNAs) can control the flux of genetic information; affect RNA stability and play crucial roles in mediating epigenetic modifications. A number of studies have highlighted the potential roles of both virus-encoded and host-encoded ncRNAs in viral infections, transmission and therapeutics. However, the role of an emerging type of non-coding transcript, circular RNA (circRNA) in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has not been fully elucidated so far. Moreover, the potential pathogenic role of circRNA-miRNA-mRNA regulatory axis has not been fully explored as yet. The current study aimed to holistically map the regulatory networks driven by SARS-CoV-2 related circRNAs, miRNAs and mRNAs to uncover plausible interactions and interplay amongst them in order to explore possible therapeutic options in SARS-CoV-2 infection. Patient datasets were analyzed systematically in a unified approach to explore circRNA, miRNA, and mRNA expression profiles. CircRNA-miRNA-mRNA network was constructed based on cytokine storm related circRNAs forming a total of 165 circRNA-miRNA-mRNA pairs. This study implies the potential regulatory role of the obtained circRNA-miRNA-mRNA network and proposes that two differentially expressed circRNAs hsa_circ_0080942 and hsa_circ_0080135 might serve as a potential theranostic agents for SARS-CoV-2 infection. Collectively, the results shed light on the functional role of circRNAs as ceRNAs to sponge miRNA and regulate mRNA expression during SARS-CoV-2 infection.