tetano
Editor, Senior Moderator
PLoS One
. 2020 Sep 9;15(9):e0238089.
doi: 10.1371/journal.pone.0238089. eCollection 2020.
Identification of immunodominant linear epitopes from SARS-CoV-2 patient plasma
Lluc Farrera-Soler[SUP] 1 [/SUP], Jean-Pierre Daguer[SUP] 1 [/SUP], Sofia Barluenga[SUP] 1 [/SUP], Oscar Vadas[SUP] 2 [/SUP], Patrick Cohen[SUP] 3 [/SUP], Sabrina Pagano[SUP] 3 [/SUP], Sabine Yerly[SUP] 3 [/SUP], Laurent Kaiser[SUP] 3 4 [/SUP], Nicolas Vuilleumier[SUP] 3 [/SUP], Nicolas Winssinger[SUP] 1 [/SUP]
Affiliations
Abstract
A novel severe acute respiratory syndrome coronavirus (SARS-CoV-2) is the source of a current pandemic (COVID-19) with devastating consequences in public health and economic stability. Using a peptide array to map the antibody response of plasma from healing patients (12) and heathy patients (6), we identified three immunodominant linear epitopes, two of which correspond to key proteolytic sites on the spike protein (S1/S2 and S2') known to be critical for cellular entry. We show biochemical evidence that plasma positive for the epitope adjacent to the S1/S2 cleavage site inhibits furin-mediated proteolysis of spike.
. 2020 Sep 9;15(9):e0238089.
doi: 10.1371/journal.pone.0238089. eCollection 2020.
Identification of immunodominant linear epitopes from SARS-CoV-2 patient plasma
Lluc Farrera-Soler[SUP] 1 [/SUP], Jean-Pierre Daguer[SUP] 1 [/SUP], Sofia Barluenga[SUP] 1 [/SUP], Oscar Vadas[SUP] 2 [/SUP], Patrick Cohen[SUP] 3 [/SUP], Sabrina Pagano[SUP] 3 [/SUP], Sabine Yerly[SUP] 3 [/SUP], Laurent Kaiser[SUP] 3 4 [/SUP], Nicolas Vuilleumier[SUP] 3 [/SUP], Nicolas Winssinger[SUP] 1 [/SUP]
Affiliations
- PMID: 32903266
- DOI: 10.1371/journal.pone.0238089
Abstract
A novel severe acute respiratory syndrome coronavirus (SARS-CoV-2) is the source of a current pandemic (COVID-19) with devastating consequences in public health and economic stability. Using a peptide array to map the antibody response of plasma from healing patients (12) and heathy patients (6), we identified three immunodominant linear epitopes, two of which correspond to key proteolytic sites on the spike protein (S1/S2 and S2') known to be critical for cellular entry. We show biochemical evidence that plasma positive for the epitope adjacent to the S1/S2 cleavage site inhibits furin-mediated proteolysis of spike.