tetano
Editor, Senior Moderator
PLoS One
. 2021 Feb 22;16(2):e0247253.
doi: 10.1371/journal.pone.0247253. eCollection 2021.
Convergent antibody evolution and clonotype expansion following influenza virus vaccination
David Forgacs[SUP] 1 [/SUP], Rodrigo B Abreu[SUP] 1 [/SUP], Giuseppe A Sautto[SUP] 1 [/SUP], Greg A Kirchenbaum[SUP] 1 [/SUP], Elliott Drabek[SUP] 2 [/SUP], Kevin S Williamson[SUP] 2 [/SUP], Dongkyoon Kim[SUP] 2 [/SUP], Daniel E Emerling[SUP] 2 [/SUP], Ted M Ross[SUP] 1 3 [/SUP]
Affiliations
Abstract
Recent advances in high-throughput single cell sequencing have opened up new avenues into the investigation of B cell receptor (BCR) repertoires. In this study, PBMCs were collected from 17 human participants vaccinated with the split-inactivated influenza virus vaccine during the 2016-2017 influenza season. A combination of Immune Repertoire Capture (IRCTM) technology and IgG sequencing was performed on ~7,800 plasmablast (PB) cells and preferential IgG heavy-light chain pairings were investigated. In some participants, a single expanded clonotype accounted for ~22% of their PB BCR repertoire. Approximately 60% (10/17) of participants experienced convergent evolution, possessing public PBs that were elicited independently in multiple participants. Binding profiles of one private and three public PBs confirmed they were all subtype-specific, cross-reactive hemagglutinin (HA) head-directed antibodies. Collectively, this high-resolution antibody repertoire analysis demonstrated the impact evolution can have on BCRs in response to influenza virus vaccination, which can guide future universal influenza prophylactic approaches.
. 2021 Feb 22;16(2):e0247253.
doi: 10.1371/journal.pone.0247253. eCollection 2021.
Convergent antibody evolution and clonotype expansion following influenza virus vaccination
David Forgacs[SUP] 1 [/SUP], Rodrigo B Abreu[SUP] 1 [/SUP], Giuseppe A Sautto[SUP] 1 [/SUP], Greg A Kirchenbaum[SUP] 1 [/SUP], Elliott Drabek[SUP] 2 [/SUP], Kevin S Williamson[SUP] 2 [/SUP], Dongkyoon Kim[SUP] 2 [/SUP], Daniel E Emerling[SUP] 2 [/SUP], Ted M Ross[SUP] 1 3 [/SUP]
Affiliations
- PMID: 33617543
- DOI: 10.1371/journal.pone.0247253
Abstract
Recent advances in high-throughput single cell sequencing have opened up new avenues into the investigation of B cell receptor (BCR) repertoires. In this study, PBMCs were collected from 17 human participants vaccinated with the split-inactivated influenza virus vaccine during the 2016-2017 influenza season. A combination of Immune Repertoire Capture (IRCTM) technology and IgG sequencing was performed on ~7,800 plasmablast (PB) cells and preferential IgG heavy-light chain pairings were investigated. In some participants, a single expanded clonotype accounted for ~22% of their PB BCR repertoire. Approximately 60% (10/17) of participants experienced convergent evolution, possessing public PBs that were elicited independently in multiple participants. Binding profiles of one private and three public PBs confirmed they were all subtype-specific, cross-reactive hemagglutinin (HA) head-directed antibodies. Collectively, this high-resolution antibody repertoire analysis demonstrated the impact evolution can have on BCRs in response to influenza virus vaccination, which can guide future universal influenza prophylactic approaches.