tetano
Editor, Senior Moderator
PLoS One
. 2021 Dec 16;16(12):e0250234.
doi: 10.1371/journal.pone.0250234. eCollection 2021.
A randomized controlled trial to evaluate the effect of influenza vaccination and probiotic supplementation on immune response and incidence of influenza-like illness in an elderly population in Indonesia
Sukamto Koesnoe[SUP] 1 2 [/SUP], Nuning Masjkuri[SUP] 3 [/SUP], Asri Adisasmita[SUP] 3 [/SUP], Samsuridjal Djauzi[SUP] 1 2 [/SUP], Cissy Kartasasmita[SUP] 4 [/SUP], Julitasari Sundoro[SUP] 5 [/SUP], Mardiati Nadjib[SUP] 6 [/SUP], Mondastri Korib[SUP] 3 [/SUP], Alisa Nurul Muthia[SUP] 7 [/SUP], Virly Nanda Muzellina[SUP] 8 9 [/SUP], Ummu Habibah[SUP] 7 [/SUP], Saskia Aziza Nursyirwan[SUP] 8 9 [/SUP], Kristoforus Hendra Djaya[SUP] 7 [/SUP], Novilia Sjafri Bachtiar[SUP] 10 [/SUP], Rini Mulia Sari[SUP] 10 [/SUP]
Affiliations
Abstract
Aim: To investigate the effect of influenza vaccination with or without probiotic supplementation on the immune response and incidence of influenza-like illness (ILI) in the elderly.
Methods: A randomized double-blind, placebo-controlled trial with a modified factorial design was conducted in 554 healthy elderly subjects aged 67 ± 5.6 (ranging from 60-90) years old in the Primary Health Care Center (Puskesmas area) of the Pulo Gadung District East Jakarta. Subjects received either a trivalent influenza vaccine or placebo at the start of the study, and a probiotic supplement (Lactobacillus helveticus R0052 and Lactobacillus rhamnosus R0011) or a placebo for 6 months. Subjects were randomly assigned into four intervention groups: influenza vaccine and probiotics (n = 141), influenza vaccine and placebo (n = 136), placebo and probiotics (n = 140), and both placebo (n = 137). The primary outcome was ILI incidence within 6 months. The secondary outcomes were seroprotection and seroconversion rates at 1, 4, and 6 months after administering the interventions.
Results: This study showed that the trivalent influenza vaccine increased seroprotection (RR 3.6 [95%CI 2.92-4.47]; p<0.010) and seroconversion (RR 29.8 [95%CI 11.1-79.5]; p<0.010) rates 1 month after vaccination in elderly people while the probiotic supplement did not alter influenza antibody titers (p = 1.000 and p = 0.210). The relative ILI incidence risk was similar between vaccinated and non-vaccinated groups, as well as in the probiotic group compared to the non-probiotic group.
Conclusion: The tested trivalent influenza vaccine significantly induced seroprotection and seroconversion in the vaccinated subjects, while probiotics administration did not influence these parameters. Vaccinated individuals displayed a similarly low ILI incidence as those in the Control Group. However, the observed trend towards a reduction of ILI incidence with probiotics supplementation warrants further assessments in a larger, at-risk population.
. 2021 Dec 16;16(12):e0250234.
doi: 10.1371/journal.pone.0250234. eCollection 2021.
A randomized controlled trial to evaluate the effect of influenza vaccination and probiotic supplementation on immune response and incidence of influenza-like illness in an elderly population in Indonesia
Sukamto Koesnoe[SUP] 1 2 [/SUP], Nuning Masjkuri[SUP] 3 [/SUP], Asri Adisasmita[SUP] 3 [/SUP], Samsuridjal Djauzi[SUP] 1 2 [/SUP], Cissy Kartasasmita[SUP] 4 [/SUP], Julitasari Sundoro[SUP] 5 [/SUP], Mardiati Nadjib[SUP] 6 [/SUP], Mondastri Korib[SUP] 3 [/SUP], Alisa Nurul Muthia[SUP] 7 [/SUP], Virly Nanda Muzellina[SUP] 8 9 [/SUP], Ummu Habibah[SUP] 7 [/SUP], Saskia Aziza Nursyirwan[SUP] 8 9 [/SUP], Kristoforus Hendra Djaya[SUP] 7 [/SUP], Novilia Sjafri Bachtiar[SUP] 10 [/SUP], Rini Mulia Sari[SUP] 10 [/SUP]
Affiliations
- PMID: 34914726
- DOI: 10.1371/journal.pone.0250234
Abstract
Aim: To investigate the effect of influenza vaccination with or without probiotic supplementation on the immune response and incidence of influenza-like illness (ILI) in the elderly.
Methods: A randomized double-blind, placebo-controlled trial with a modified factorial design was conducted in 554 healthy elderly subjects aged 67 ± 5.6 (ranging from 60-90) years old in the Primary Health Care Center (Puskesmas area) of the Pulo Gadung District East Jakarta. Subjects received either a trivalent influenza vaccine or placebo at the start of the study, and a probiotic supplement (Lactobacillus helveticus R0052 and Lactobacillus rhamnosus R0011) or a placebo for 6 months. Subjects were randomly assigned into four intervention groups: influenza vaccine and probiotics (n = 141), influenza vaccine and placebo (n = 136), placebo and probiotics (n = 140), and both placebo (n = 137). The primary outcome was ILI incidence within 6 months. The secondary outcomes were seroprotection and seroconversion rates at 1, 4, and 6 months after administering the interventions.
Results: This study showed that the trivalent influenza vaccine increased seroprotection (RR 3.6 [95%CI 2.92-4.47]; p<0.010) and seroconversion (RR 29.8 [95%CI 11.1-79.5]; p<0.010) rates 1 month after vaccination in elderly people while the probiotic supplement did not alter influenza antibody titers (p = 1.000 and p = 0.210). The relative ILI incidence risk was similar between vaccinated and non-vaccinated groups, as well as in the probiotic group compared to the non-probiotic group.
Conclusion: The tested trivalent influenza vaccine significantly induced seroprotection and seroconversion in the vaccinated subjects, while probiotics administration did not influence these parameters. Vaccinated individuals displayed a similarly low ILI incidence as those in the Control Group. However, the observed trend towards a reduction of ILI incidence with probiotics supplementation warrants further assessments in a larger, at-risk population.