tetano
Editor, Senior Moderator
Virology. 2016 Jun 13;496:131-137. doi: 10.1016/j.virol.2016.06.003. [Epub ahead of print]
[h=1]PLC-γ1 is involved in the inflammatory response induced by influenza A virus H1N1 infection.[/h] Zhu L[SUP]1[/SUP], Yuan C[SUP]1[/SUP], Ding X[SUP]1[/SUP], Xu S[SUP]2[/SUP], Yang J[SUP]2[/SUP], Liang Y[SUP]3[/SUP], Zhu Q[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] We have previously reported that phosphoinositide-specific phospholipase γ1 (PLC-γ1) signaling is activated by influenza virus H1N1 infection and mediates efficient viral entry in human epithelial cells. In this study, we show that H1N1 also activates PLCγ-1 signaling in human promonocytic cell line -derived macrophages. Surprisingly, the activated PLCγ-1 signaling is not important for viral replication in macrophages, but is involved in the virus-induced inflammatory responses. PLC-γ1-specific inhibitor U73122 strongly inhibits the H1N1 virus-induced NF-κB signaling, blocking the up-regulation of TNF-α, IL-6, MIP-1α, and reactive oxidative species. In a positive feedback loop, IL-1β and TNF-α activate the PLCγ-1 signaling in both epithelial and macrophage cell lines. In summary, we have shown for the first time that the PLCγ-1 signaling plays an important role in the H1N1-induced inflammatory responses. Our study suggests that targeting the PLCγ-1 signaling is a potential antiviral therapy against H1N1 by inhibiting both viral replication and excessive inflammation.
Copyright ? 2016 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] H1N1; Inflammation; Influenza A virus; Macrophage; PLC-γ1; Pro-inflammatory cytokines; ROS
PMID: 27310357 [PubMed - as supplied by publisher]
[h=1]PLC-γ1 is involved in the inflammatory response induced by influenza A virus H1N1 infection.[/h] Zhu L[SUP]1[/SUP], Yuan C[SUP]1[/SUP], Ding X[SUP]1[/SUP], Xu S[SUP]2[/SUP], Yang J[SUP]2[/SUP], Liang Y[SUP]3[/SUP], Zhu Q[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] We have previously reported that phosphoinositide-specific phospholipase γ1 (PLC-γ1) signaling is activated by influenza virus H1N1 infection and mediates efficient viral entry in human epithelial cells. In this study, we show that H1N1 also activates PLCγ-1 signaling in human promonocytic cell line -derived macrophages. Surprisingly, the activated PLCγ-1 signaling is not important for viral replication in macrophages, but is involved in the virus-induced inflammatory responses. PLC-γ1-specific inhibitor U73122 strongly inhibits the H1N1 virus-induced NF-κB signaling, blocking the up-regulation of TNF-α, IL-6, MIP-1α, and reactive oxidative species. In a positive feedback loop, IL-1β and TNF-α activate the PLCγ-1 signaling in both epithelial and macrophage cell lines. In summary, we have shown for the first time that the PLCγ-1 signaling plays an important role in the H1N1-induced inflammatory responses. Our study suggests that targeting the PLCγ-1 signaling is a potential antiviral therapy against H1N1 by inhibiting both viral replication and excessive inflammation.
Copyright ? 2016 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] H1N1; Inflammation; Influenza A virus; Macrophage; PLC-γ1; Pro-inflammatory cytokines; ROS
PMID: 27310357 [PubMed - as supplied by publisher]