tetano
Editor, Senior Moderator
Vaccine. 2015 Sep 30. pii: S0264-410X(15)01341-9. doi: 10.1016/j.vaccine.2015.09.065. [Epub ahead of print]
[h=1]Plant-derived H7 VLP vaccine elicits protective immune response against H7N9 influenza virus in mice and ferrets.[/h] Pillet S[SUP]1[/SUP], Racine T[SUP]2[/SUP], Nfon C[SUP]3[/SUP], Di Lenardo TZ[SUP]4[/SUP], Babiuk S[SUP]5[/SUP], Ward BJ[SUP]6[/SUP], Kobinger GP[SUP]7[/SUP], Landry N[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] In March 2013, the Chinese Centre for Disease Control and Prevention confirmed the first reported case of human infection with an avian influenza A H7N9 virus. Infection with this virus often caused severe pneumonia and acute respiratory distress syndrome resulting in a case fatality rate >35%. The risk of pandemic highlighted, once again, the need for a more rapid and scalable vaccine response capability. Here, we describe the rapid (19 days) development of a plant-derived VLP vaccine based on the hemagglutinin sequence of influenza H7N9 A/Hangzhou/1/2013. The immunogenicity of the H7 VLP vaccine was assessed in mice and ferrets after one or two intramuscular dose(s) with and without adjuvant (alum or GLA-SE?). In ferrets, we also measured H7-specific cell-mediated immunity. The mice and ferrets were then challenged with H7N9 A/Anhui/1/2013 influenza virus. A single immunization with the adjuvanted vaccine elicited a strong humoral response and protected mice against an otherwise lethal challenge. Two doses of unadjuvanted vaccine significantly increased humoral response and resulted in 100% protection with significant reduction of clinical signs leading to nearly asymptomatic infections. In ferrets, a single immunization with the alum-adjuvanted H7 VLP vaccine induced strong humoral and CMI responses with antigen-specific activation of CD3[SUP]+[/SUP] T cells. Compared to animals injected with placebo, ferrets vaccinated with alum-adjuvanted vaccine displayed no weight loss during the challenge. Moreover, the vaccination significantly reduced the viral load in lungs and nasal washes 3 days after the infection. This candidate plant-made H7 vaccine therefore induced protective responses after either one adjuvanted or two unadjuvanted doses. Studies are currently ongoing to better characterize the immune response elicited by the plant-derived VLP vaccines. Regardless, these data are very promising for the rapid production of an immunogenic and protective vaccine against this potentially pandemic virus.
Copyright ? 2015. Published by Elsevier Ltd.
[h=4]KEYWORDS:[/h] Ferrets; H7N9; Immunogenicity; Influenza A; Mice; NICOTIANA benthamiana; Plant-made vaccine; Virus-like particle
PMID: 26432915 [PubMed - as supplied by publisher]
[h=1]Plant-derived H7 VLP vaccine elicits protective immune response against H7N9 influenza virus in mice and ferrets.[/h] Pillet S[SUP]1[/SUP], Racine T[SUP]2[/SUP], Nfon C[SUP]3[/SUP], Di Lenardo TZ[SUP]4[/SUP], Babiuk S[SUP]5[/SUP], Ward BJ[SUP]6[/SUP], Kobinger GP[SUP]7[/SUP], Landry N[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] In March 2013, the Chinese Centre for Disease Control and Prevention confirmed the first reported case of human infection with an avian influenza A H7N9 virus. Infection with this virus often caused severe pneumonia and acute respiratory distress syndrome resulting in a case fatality rate >35%. The risk of pandemic highlighted, once again, the need for a more rapid and scalable vaccine response capability. Here, we describe the rapid (19 days) development of a plant-derived VLP vaccine based on the hemagglutinin sequence of influenza H7N9 A/Hangzhou/1/2013. The immunogenicity of the H7 VLP vaccine was assessed in mice and ferrets after one or two intramuscular dose(s) with and without adjuvant (alum or GLA-SE?). In ferrets, we also measured H7-specific cell-mediated immunity. The mice and ferrets were then challenged with H7N9 A/Anhui/1/2013 influenza virus. A single immunization with the adjuvanted vaccine elicited a strong humoral response and protected mice against an otherwise lethal challenge. Two doses of unadjuvanted vaccine significantly increased humoral response and resulted in 100% protection with significant reduction of clinical signs leading to nearly asymptomatic infections. In ferrets, a single immunization with the alum-adjuvanted H7 VLP vaccine induced strong humoral and CMI responses with antigen-specific activation of CD3[SUP]+[/SUP] T cells. Compared to animals injected with placebo, ferrets vaccinated with alum-adjuvanted vaccine displayed no weight loss during the challenge. Moreover, the vaccination significantly reduced the viral load in lungs and nasal washes 3 days after the infection. This candidate plant-made H7 vaccine therefore induced protective responses after either one adjuvanted or two unadjuvanted doses. Studies are currently ongoing to better characterize the immune response elicited by the plant-derived VLP vaccines. Regardless, these data are very promising for the rapid production of an immunogenic and protective vaccine against this potentially pandemic virus.
Copyright ? 2015. Published by Elsevier Ltd.
[h=4]KEYWORDS:[/h] Ferrets; H7N9; Immunogenicity; Influenza A; Mice; NICOTIANA benthamiana; Plant-made vaccine; Virus-like particle
PMID: 26432915 [PubMed - as supplied by publisher]