tetano
Editor, Senior Moderator
Eur J Pharm Biopharm. 2013 Aug 7. pii: S0939-6411(13)00278-6. doi: 10.1016/j.ejpb.2013.07.018. [Epub ahead of print]
Physical and immunogenic stability of spray freeze dried influenza vaccine powder for pulmonary delivery: comparison of inulin, dextran or a mixture of dextran and trehalose as protectants.
Murugappan S, Patil HP, Kanojia G, Veer WT, Meijerhof T, Frijlink HW, Huckriede A, Hinrichs WL.
Source
Department of Pharmaceutical Technology and Biopharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV, Groningen, The Netherlands. Electronic address: s.murugappan@rug.nl.
Abstract
One of the advantages of dry influenza vaccines over conventional liquid influenza vaccines is that they can be used for alternative routes of administration. Previous studies showed that spray freeze drying is an excellent technique to prepare vaccine containing powders for pulmonary delivery (Amorij,J-P.; et al, 2007; Audouy,S.A.; et al, 2011). The aim of this study was to investigate the physical and immunogenic stability of spray freeze-dried whole inactivated virus influenza vaccine prepared by using inulin, dextran and a mixture of dextran and trehalose as protectants. Physical and biochemical characteristics of the vaccine powder were maintained at temperatures up to 30 ?C for three months. In addition, in vivo data indicate that also the immunogenic properties of the vaccine were maintained under these storage conditions. On the other hand, in vivo results also revealed that subtle changes in powder characteristics were induced during storage at 30 ?C. However, laser diffraction measurements showed that problems associated with these subtle changes can be overcome by using dry powder inhalers with an efficient powder dispersing capacity.
Copyright ? 2013. Published by Elsevier B.V.
KEYWORDS:
Antigen stability, Dextran, Influenza vaccine, Inulin, Physical stability, Pulmonary powder, Trehalose
PMID:
23933147
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23933147
Physical and immunogenic stability of spray freeze dried influenza vaccine powder for pulmonary delivery: comparison of inulin, dextran or a mixture of dextran and trehalose as protectants.
Murugappan S, Patil HP, Kanojia G, Veer WT, Meijerhof T, Frijlink HW, Huckriede A, Hinrichs WL.
Source
Department of Pharmaceutical Technology and Biopharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV, Groningen, The Netherlands. Electronic address: s.murugappan@rug.nl.
Abstract
One of the advantages of dry influenza vaccines over conventional liquid influenza vaccines is that they can be used for alternative routes of administration. Previous studies showed that spray freeze drying is an excellent technique to prepare vaccine containing powders for pulmonary delivery (Amorij,J-P.; et al, 2007; Audouy,S.A.; et al, 2011). The aim of this study was to investigate the physical and immunogenic stability of spray freeze-dried whole inactivated virus influenza vaccine prepared by using inulin, dextran and a mixture of dextran and trehalose as protectants. Physical and biochemical characteristics of the vaccine powder were maintained at temperatures up to 30 ?C for three months. In addition, in vivo data indicate that also the immunogenic properties of the vaccine were maintained under these storage conditions. On the other hand, in vivo results also revealed that subtle changes in powder characteristics were induced during storage at 30 ?C. However, laser diffraction measurements showed that problems associated with these subtle changes can be overcome by using dry powder inhalers with an efficient powder dispersing capacity.
Copyright ? 2013. Published by Elsevier B.V.
KEYWORDS:
Antigen stability, Dextran, Influenza vaccine, Inulin, Physical stability, Pulmonary powder, Trehalose
PMID:
23933147
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23933147