tetano
Editor, Senior Moderator
Proc Natl Acad Sci U S A. 2017 Jan 17. pii: 201617959. doi: 10.1073/pnas.1617959114. [Epub ahead of print]
[h=1]Phylogenetic analysis of the human antibody repertoire reveals quantitative signatures of immune senescence and aging.[/h] de Bourcy CF[SUP]1[/SUP], Angel CJ[SUP]2[/SUP], Vollmers C[SUP]3[/SUP], Dekker CL[SUP]4[/SUP], Davis MM[SUP]2,[/SUP][SUP]5,[/SUP][SUP]6[/SUP], Quake SR[SUP]7,[/SUP][SUP]3,[/SUP][SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The elderly have reduced humoral immunity, as manifested by increased susceptibility to infections and impaired vaccine responses. To investigate the effects of aging on B-cell receptor (BCR) repertoire evolution during an immunological challenge, we used a phylogenetic distance metric to analyze Ig heavy-chain transcript sequences in both young and elderly individuals before and after influenza vaccination. We determined that BCR repertoires become increasingly specialized over a span of decades, but less plastic. In 50% of the elderly individuals, a large space in the repertoire was occupied by a small number of recall lineages that did not decline during vaccine response and contained hypermutated IgD[SUP]+[/SUP] B cells. Relative to their younger counterparts, older subjects demonstrated a contracted naive repertoire and diminished intralineage diversification, signifying a reduced substrate for mounting novel responses and decreased fine-tuning of BCR specificities by somatic hypermutation. Furthermore, a larger proportion of the repertoire exhibited premature stop codons in some elderly subjects, indicating that aging may negatively affect the ability of B cells to discriminate between functional and nonfunctional receptors. Finally, we observed a decreased incidence of radical mutations compared with conservative mutations in elderly subjects' vaccine responses, which suggests that accumulating original antigenic sin may be limiting the accessible space for paratope evolution. Our findings shed light on the complex interplay of environmental and gerontological factors affecting immune senescence, and provide direct molecular characterization of the effects of senescence on the immune repertoire.
[h=4]KEYWORDS:[/h] CMV; UniFrac; aging; antibody repertoire; influenza vaccine
PMID: 28096374 DOI: 10.1073/pnas.1617959114
[PubMed - as supplied by publisher]
[h=1]Phylogenetic analysis of the human antibody repertoire reveals quantitative signatures of immune senescence and aging.[/h] de Bourcy CF[SUP]1[/SUP], Angel CJ[SUP]2[/SUP], Vollmers C[SUP]3[/SUP], Dekker CL[SUP]4[/SUP], Davis MM[SUP]2,[/SUP][SUP]5,[/SUP][SUP]6[/SUP], Quake SR[SUP]7,[/SUP][SUP]3,[/SUP][SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The elderly have reduced humoral immunity, as manifested by increased susceptibility to infections and impaired vaccine responses. To investigate the effects of aging on B-cell receptor (BCR) repertoire evolution during an immunological challenge, we used a phylogenetic distance metric to analyze Ig heavy-chain transcript sequences in both young and elderly individuals before and after influenza vaccination. We determined that BCR repertoires become increasingly specialized over a span of decades, but less plastic. In 50% of the elderly individuals, a large space in the repertoire was occupied by a small number of recall lineages that did not decline during vaccine response and contained hypermutated IgD[SUP]+[/SUP] B cells. Relative to their younger counterparts, older subjects demonstrated a contracted naive repertoire and diminished intralineage diversification, signifying a reduced substrate for mounting novel responses and decreased fine-tuning of BCR specificities by somatic hypermutation. Furthermore, a larger proportion of the repertoire exhibited premature stop codons in some elderly subjects, indicating that aging may negatively affect the ability of B cells to discriminate between functional and nonfunctional receptors. Finally, we observed a decreased incidence of radical mutations compared with conservative mutations in elderly subjects' vaccine responses, which suggests that accumulating original antigenic sin may be limiting the accessible space for paratope evolution. Our findings shed light on the complex interplay of environmental and gerontological factors affecting immune senescence, and provide direct molecular characterization of the effects of senescence on the immune repertoire.
[h=4]KEYWORDS:[/h] CMV; UniFrac; aging; antibody repertoire; influenza vaccine
PMID: 28096374 DOI: 10.1073/pnas.1617959114
[PubMed - as supplied by publisher]