tetano
Editor, Senior Moderator
Appl Environ Microbiol. 2016 Mar 18. pii: AEM.04046-15. [Epub ahead of print]
[h=1]Persistence of Influenza A (H1N1) Virus on Stainless Steel Surfaces.[/h] Perry KA[SUP]1[/SUP], Coulliette AD[SUP]2[/SUP], Rose LJ[SUP]3[/SUP], Shams AM[SUP]3[/SUP], Edwards JR[SUP]3[/SUP], Noble-Wang JA[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] As annual influenza epidemics continue to cause significant morbidity and economic burden, an understanding of viral persistence and transmission is critical for public health officials and healthcare workers to better protect patients and their family members from infection. The infectivity and persistence of two influenza A (H1N1) strains (A/New Caledonia/20/1999 and A/Brisbane/59/2007) were evaluated on stainless steel (SS) surfaces using three different surfaces matrices (2% fetal bovine serum, 5 mg/mL mucin, and viral medium) at varying absolute humidity conditions (4.1 x 10[SUP]5[/SUP] mPa, 6.5 x 10[SUP]5[/SUP] mPa, 7.1 x 10[SUP]5[/SUP] mPa, 11.4 x 10[SUP]5[/SUP] mPa, 11.2 x 10[SUP]5[/SUP] mPa, and 17.9 x 10[SUP]5[/SUP] mPa) for up to seven days. Influenza virus was deposited onto SS coupons (7.07 cm[SUP]2[/SUP]) and recovered by agitation and sonicating in viral medium. Viral persistence was quantified using a tissue culture based enzyme-linked immunosorbent assay (ELISA) to determine the median tissue culture infective dose (TCID[SUB]50[/SUB]) of infectious virus per coupon. Overall, both strains of influenza A virus remained infectious on SS coupons with an approximate 2 log[SUB]10[/SUB] loss over seven days. Factors that influenced viral persistence included absolute humidity, strain/absolute humidity interaction, and time (P ≤ 0.01). Further studies into hand transfer of influenza A virus from fomites and the impact of inanimate surface contamination in transmission should be investigated as this study demonstrates prolonged persistence on non-porous surfaces.
[h=4]IMPORTANCE:[/h] The study tested the ability of two influenza A H1N1 strains to persist and remain infectious on stainless steel surfaces in varying environmental conditions. It is demonstrated that influenza A H1N1 virus can persist and remain infectious on stainless steel surfaces for 7 days. This raises the question of what role contaminated surfaces play in the transmission of influenza A virus and that additional studies should be conducted to assess this.
Copyright ? 2016, American Society for Microbiology. All Rights Reserved.
PMID: 26994082 [PubMed - as supplied by publisher]
[h=1]Persistence of Influenza A (H1N1) Virus on Stainless Steel Surfaces.[/h] Perry KA[SUP]1[/SUP], Coulliette AD[SUP]2[/SUP], Rose LJ[SUP]3[/SUP], Shams AM[SUP]3[/SUP], Edwards JR[SUP]3[/SUP], Noble-Wang JA[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] As annual influenza epidemics continue to cause significant morbidity and economic burden, an understanding of viral persistence and transmission is critical for public health officials and healthcare workers to better protect patients and their family members from infection. The infectivity and persistence of two influenza A (H1N1) strains (A/New Caledonia/20/1999 and A/Brisbane/59/2007) were evaluated on stainless steel (SS) surfaces using three different surfaces matrices (2% fetal bovine serum, 5 mg/mL mucin, and viral medium) at varying absolute humidity conditions (4.1 x 10[SUP]5[/SUP] mPa, 6.5 x 10[SUP]5[/SUP] mPa, 7.1 x 10[SUP]5[/SUP] mPa, 11.4 x 10[SUP]5[/SUP] mPa, 11.2 x 10[SUP]5[/SUP] mPa, and 17.9 x 10[SUP]5[/SUP] mPa) for up to seven days. Influenza virus was deposited onto SS coupons (7.07 cm[SUP]2[/SUP]) and recovered by agitation and sonicating in viral medium. Viral persistence was quantified using a tissue culture based enzyme-linked immunosorbent assay (ELISA) to determine the median tissue culture infective dose (TCID[SUB]50[/SUB]) of infectious virus per coupon. Overall, both strains of influenza A virus remained infectious on SS coupons with an approximate 2 log[SUB]10[/SUB] loss over seven days. Factors that influenced viral persistence included absolute humidity, strain/absolute humidity interaction, and time (P ≤ 0.01). Further studies into hand transfer of influenza A virus from fomites and the impact of inanimate surface contamination in transmission should be investigated as this study demonstrates prolonged persistence on non-porous surfaces.
[h=4]IMPORTANCE:[/h] The study tested the ability of two influenza A H1N1 strains to persist and remain infectious on stainless steel surfaces in varying environmental conditions. It is demonstrated that influenza A H1N1 virus can persist and remain infectious on stainless steel surfaces for 7 days. This raises the question of what role contaminated surfaces play in the transmission of influenza A virus and that additional studies should be conducted to assess this.
Copyright ? 2016, American Society for Microbiology. All Rights Reserved.
PMID: 26994082 [PubMed - as supplied by publisher]